Integrative multi-omics reveals genome evolution and CYP719-mediated BIA biosynthesis in Tinospora sagittata

IF 5.1 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2026-03-01 Epub Date: 2026-03-11 DOI:10.1016/j.cpb.2026.100602

Mohammad Murtaza Alami , Shaohua Shu , Sanbo Liu , Shengqiu Feng , Guozheng Yang , Zhinan Mei , Xuekui Wang

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引用次数: 0

Abstract

Tinospora sagittata (Oliv.) Gagnep. is an essential medicinal tetraploid plant in the Menispermaceae family. Its tuber, “Radix Tinosporae,” is widely used in Traditional Chinese Medicine and is rich in terpenoids and benzylisoquinoline alkaloids (BIAs). To better understand the biosynthesis of these compounds and the evolution of the T. sagittata genome, we performed comparative genomics with 16 other plant species, estimating its evolutionary placement and divergence time within Ranunculales. Genome evolution analyses revealed one round of tandem duplication approximately 1.5 million years ago and one whole-genome duplication (WGD) around 86.9 Mya. WGD contributed to the expansion of the clade-specific cytochrome P450 gene families in Ranunculales. Genome-wide mining identified genes involved in BIA biosynthesis, and transcriptomic profiling was combined with targeted and untargeted metabolomics to analyze gene expression and metabolite accumulation. Finally, one CYP719 gene candidate (TsA02G014550) was functionally characterized to catalyze the formation of (S)-canadine in the jatrorrhizine biosynthetic pathway. Our integrative genomics, transcriptomics, and metabolomics analyses provide new insights into the evolution of the T. sagittata genome and BIA biosynthesis, supporting future sustainable production of these valuable secondary metabolites.

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整合多组学揭示箭状木霉基因组进化和cyp719介导的BIA生物合成

矢状小孢子虫（橄榄）Gagnep。是一种重要的药用四倍体植物。其块茎“Tinosporae”被广泛用于中药，富含萜类和苯并异喹啉生物碱（BIAs）。为了更好地了解这些化合物的生物合成和箭形霉基因组的进化，我们与其他16个植物物种进行了比较基因组学研究，估计了其在毛茛属植物中的进化位置和分化时间。基因组进化分析揭示了大约150万年前的一轮串联重复和大约86.9亿年前的一次全基因组重复。WGD有助于毛茛属植物枝特异性细胞色素P450基因家族的扩展。全基因组挖掘鉴定了参与BIA生物合成的基因，转录组学分析结合靶向和非靶向代谢组学分析基因表达和代谢物积累。最后，对一个CYP719候选基因（TsA02G014550）进行了功能表征，在麻草酸生物合成途径中催化(S)-canadine的形成。我们的综合基因组学、转录组学和代谢组学分析为弓形虫基因组的进化和BIA的生物合成提供了新的见解，为这些有价值的次生代谢物的未来可持续生产提供了支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.