Comparison of Antimicrobial and Antibiofilm Properties of a New Endodontic Sealer against Enterococcus faecalis with AH Plus Sealer.

Abstract

Introduction: This study aimed to evaluate the antimicrobial and antibiofilm properties of a novel endodontic sealer against Enterococcus faecalis (E. faecalis). Long-lasting antimicrobial sealers can inhibit bacterial growth and reduce persistent infections following root canal treatment. The primary objective was to compare the new sealer with the AH Plus sealer in terms of antimicrobial and antibiofilm efficacy.

Methods: Five experimental sealer formulations (Groups A-E) were prepared using the spatula mixing method. Disks (6 mm diameter) were fabricated and evaluated using colony-forming unit (CFU) counts and disk diffusion assays. Biofilm formation by E. faecalis was assessed prior to testing the antibiofilm activity, which was quantified using the minimum biofilm inhibitory concentration (MBIC).

Results: Group D and AH Plus sealer demonstrated the lowest CFU counts among the new sealer formulations (P>0.05). Group D exhibited the largest inhibition zone compared to the other experimental groups (P>0.05). The mean MBIC against E. faecalis was 256 μg/mL for both Group D and AH Plus sealer.

Discussion: The newly developed sealer (Group D) displayed antimicrobial and antibiofilm effects comparable to AH Plus sealer against E. faecalis.

Conclusion: Group D shows promise as a candidate for further evaluation in clinical studies, demonstrating efficacy similar to the commercially available AH Plus sealer.