Blood mucorales quantitative PCR ready for prime time.

IF 4.5 3区医学 Q2 INFECTIOUS DISEASES

Current Opinion in Infectious Diseases Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI:10.1097/QCO.0000000000001161

Laurence Millon, Julie Rousselot, Emeline Scherer, Anne-Pauline Bellanger

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引用次数: 0

Abstract

Purpose of review: Mucorales quantitative PCR (qPCR) was first developed and evaluated in 2013, and since then, substantial evidence has accumulated supporting its clinical utility. This review highlights the major advances this technique has brought to the management of mucormycosis.

Recent findings: Different in-house and commercial assays are now available, targeting a broad range of clinically relevant Mucorales species. Performance depends strongly on preanalytical parameters such as volume of plasma or serum used for DNA extraction, and ratio of DNA input in the PCR reaction mix. Multiplex assays have expanded diagnostic scope to include Aspergillus and other molds, supporting differential diagnosis of invasive mold infections. Serial qPCR monitoring provides prognostic information, with DNA clearance after treatment associated with improved survival. Routine screening in high-risk populations, including hematology and severely burned patients, shortens diagnostic delays and improves outcomes.

Summary: Compared with conventional methods, Mucorales qPCR provides a rapid, noninvasive, and sensitive diagnostic tool. Large studies have shown that it enables earlier detection, facilitates timely antifungal treatment, and is associated with improved survival.

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血液粘膜定量PCR已准备就绪。

综述目的：毛霉菌定量PCR （Mucorales quantitative PCR, qPCR）于2013年首次被开发和评估，从那时起，大量证据积累支持其临床应用。本文综述了该技术在毛霉病治疗中的主要进展。最近的发现：现在有不同的内部和商业分析，针对广泛的临床相关的毛霉菌物种。性能在很大程度上取决于分析前参数，如用于DNA提取的血浆或血清的体积，以及PCR反应混合物中DNA输入的比例。多重检测扩大了诊断范围，包括曲霉和其他霉菌，支持侵袭性霉菌感染的鉴别诊断。序列qPCR监测提供预后信息，治疗后DNA清除与生存率提高相关。在高危人群中进行常规筛查，包括血液学和严重烧伤患者，可以缩短诊断延误并改善结果。摘要：与传统方法相比，Mucorales qPCR提供了一种快速、无创、灵敏的诊断工具。大型研究表明，它可以早期发现，促进及时的抗真菌治疗，并与提高生存率有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Opinion in Infectious Diseases 医学-传染病学

CiteScore

6.70

自引率

2.60%

发文量

121

审稿时长

6-12 weeks

期刊介绍： This reader-friendly, bimonthly resource provides a powerful, broad-based perspective on the most important advances from throughout the world literature. Featuring renowned guest editors and focusing exclusively on two topics, every issue of Current Opinion in Infectious Disease delivers unvarnished, expert assessments of developments from the previous year. Insightful editorials and on-the-mark invited reviews cover key subjects such as HIV infection and AIDS; skin and soft tissue infections; respiratory infections; paediatric and neonatal infections; gastrointestinal infections; tropical and travel-associated diseases; and antimicrobial agents.