{"title":"Continuous Crystallization Platforms to Crystallize Biomolecules.","authors":"Siyu Pu, Kunn Hadinoto","doi":"10.1007/10_2025_295","DOIUrl":null,"url":null,"abstract":"<p><p>In the realm of industrial crystallization for biomolecules, batch crystallizers have been the predominant platform, although continuous crystallizers with clearly superior performances have been actively investigated in recent years. Among them, mixed-suspension-mixed-product-removal crystallizer (MSMPRC), plug flow crystallizer (PFC), slug flow crystallizer (SFC), oscillatory flow baffled crystallizer (OFBC), and other innovative continuous platforms such as membrane crystallizer and Couette-Taylor (CT) crystallizer are introduced in this chapter. These crystallizers have been utilized in continuous crystallization of biomolecules including globular proteins such as lysozyme and insulin. Their key design variables encompassing variables affecting kinetics (residence time distribution (RTD), mixing strategy, etc.) and variables affecting thermodynamics (driving force of crystallization) are discussed. As a promising alternative to conventional chromatography method, continuous crystallization shows great potential for downstream processing of biomolecules.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"149-187"},"PeriodicalIF":0.0000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in biochemical engineering/biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/10_2025_295","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
In the realm of industrial crystallization for biomolecules, batch crystallizers have been the predominant platform, although continuous crystallizers with clearly superior performances have been actively investigated in recent years. Among them, mixed-suspension-mixed-product-removal crystallizer (MSMPRC), plug flow crystallizer (PFC), slug flow crystallizer (SFC), oscillatory flow baffled crystallizer (OFBC), and other innovative continuous platforms such as membrane crystallizer and Couette-Taylor (CT) crystallizer are introduced in this chapter. These crystallizers have been utilized in continuous crystallization of biomolecules including globular proteins such as lysozyme and insulin. Their key design variables encompassing variables affecting kinetics (residence time distribution (RTD), mixing strategy, etc.) and variables affecting thermodynamics (driving force of crystallization) are discussed. As a promising alternative to conventional chromatography method, continuous crystallization shows great potential for downstream processing of biomolecules.
期刊介绍：
Advances in Biochemical Engineering/Biotechnology reviews actual trends in modern biotechnology. Its aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required for chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. They give the state-of-the-art of a topic in a comprehensive way thus being a valuable source for the next 3 - 5 years. It also discusses new discoveries and applications.
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