Biocontrol of Chrysanthemum wilt by Bacillus velezensis HSX-1 and its antifungal mechanism

IF 4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2026-03-01 Epub Date: 2026-02-19 DOI:10.1016/j.biocontrol.2026.105993

Jian Wang, Yaping Wang, Wenchao Yu, Jiayun Chen, Yangang Dai, Zikang Li, Hang Wu, Xiaoqian Hu, Shufeng Bi

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Abstract

Chrysanthemum wilt caused by Fusarium oxysporum is a devastating disease that causes substantial economic losses. Biological control agents are considered an effective approach to control plant diseases. In the present study, the biocontrol efficacy and mechanisms of the newly isolated Bacillus velezensis strain HSX-1 against F. oxysporum were evaluated. B. velezensis HSX-1 significantly suppressed the mycelial growth of F. oxysporum by 65.52% in the dual culture test. In addition, a 15% concentration of the cell-free supernatant (CFS) from B. velezensis HSX-1 significantly suppressed colony radial growth as well as spore germination of F. oxysporum. Whole-genome sequencing identified gene clusters responsible for biocontrol activity, including those encoding nonribosomal peptides, polyketide antibiotics, siderophores, and extracellular hydrolases. B. velezensis HSX-1 has the ability to effectively reduce the virulence of F. oxysporum and promoted the growth of Chrysanthemum morifolium. Strain HSX-1 produces multiple plant growth-promoting metabolites, including indole-3-acetic acid (IAA) and siderophores. Phenotypic and transcriptome analyses revealed that strain HSX-1 inhibits F. oxysporum by disrupting the cell membrane integrity and reactive oxygen species (ROS) scavenging system. All of these results indicated that B. velezensis HSX-1 has strong potential to be developed as a biocontrol agent to control Chrysanthemum wilt caused by F. oxysporum and promote C. morifolium growth.

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芽孢杆菌HSX-1对菊花枯萎病的防制及其抑菌机制研究

由尖孢镰刀菌引起的菊花枯萎病是一种造成巨大经济损失的毁灭性疾病。生物防治剂被认为是防治植物病害的有效手段。本研究对新分离的velezensis芽孢杆菌HSX-1菌株对尖孢镰刀菌的生物防治效果及机制进行了评价。在双培养试验中，B. velezensis HSX-1对尖孢镰刀菌菌丝生长的抑制率为65.52%。此外，15%浓度的无细胞上清液（CFS）显著抑制了镰刀菌菌落径向生长和孢子萌发。全基因组测序确定了负责生物防治活性的基因簇，包括编码非核糖体肽、聚酮类抗生素、铁载体和细胞外水解酶的基因簇。B. velezensis HSX-1能有效降低尖孢镰刀菌的毒力，促进菊花的生长。菌株HSX-1产生多种促进植物生长的代谢物，包括吲哚-3-乙酸（IAA）和铁载体。表型和转录组分析表明，菌株HSX-1通过破坏细胞膜完整性和活性氧（ROS）清除系统来抑制尖孢镰刀菌。上述结果表明，白僵菌HSX-1具有很强的开发潜力，可作为防治尖孢镰刀菌引起的菊花枯萎病和促进morifolium生长的生物防治剂。

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.