Production of high-valued D-allulose from sucrose via in vitro multi-enzyme cascade

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI:10.1016/j.bej.2026.110158

Tian Xiao , Wei Zhao , Ruijin Yang , Xiaomei Lyu

引用次数: 0

Abstract

D-allulose is a prominent functional rare sugar with extensive applications in the food, cosmetic, and pharmaceutical fields. However, its commercial production approach via D-allulose 3-epimerase suffers from low conversion yield. In this study, a novel in vitro multi-enzyme cascade pathway, consisting of hydrolysis, isomerization, phosphorylation, and dephosphorylation, was constructed for high conversion of sucrose to D-allulose. By optimizing the reaction conditions and catalytic pathways, we achieved a D-allulose yield of 70.2% from 10 g/L sucrose. To investigate its potential in industrial catalysis, the sucrose concentration was increased to 50 g/L and resulted in a yield of 66.44% D-allulose. This study provides an efficient and cost-effective approach for producing D-allulose from sucrose.

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体外多酶级联酶法从蔗糖制备高价值D-allulose

D-allulose是一种重要的功能性稀有糖，在食品、化妆品和制药等领域有着广泛的应用。然而，通过D-allulose 3- epimase进行商业化生产的方法存在转化率低的问题。本研究构建了一种由水解、异构化、磷酸化和去磷酸化组成的体外多酶级联途径，以实现蔗糖向D-allulose的高转化。通过优化反应条件和催化途径，以10 g/L蔗糖为原料，D-allulose的产率达到70.2%。为考察其工业催化潜力，将蔗糖浓度提高到50 g/L， D-allulose的产率为66.44%。本研究为从蔗糖中提取D-allulose提供了一种高效、经济的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.