Simultaneous removal of nitrate, zinc, and bisphenol A by an iron-modified biochar composite mycelial pellet bioreactor: Optimization and microbial mechanisms

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-10 DOI:10.1016/j.bej.2026.110161

Yinan Wang , Jiayao Ma , Junfeng Su , Yu Liu , Li Luo , Yihan Bai , Xuan Li

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引用次数: 0

Abstract

In this study, iron-modified biochar composite mycelial pellets (CMPs) were utilized to immobilize the Fe^2 + oxidizing denitrifying bacterial strain Zoogloea sp. FY-6, with the aim of simultaneous removal of nitrate (NO₃^--N), zinc (Zn²⁺) and bisphenol A (BPA). Under conditions of 8 h hydraulic retention time (HRT), pH 7.0, and influent concentrations of 25.0 mg L⁻¹ NO₃^--N, 1.0 mg L⁻¹ Zn²⁺, and 1.0 mg L⁻¹ BPA, the bioreactor reached its best performance, with NO₃^--N, Zn²⁺, and BPA removal efficiencies of 87.2%, 92.4%, and 94.4%, respectively. The experimental results demonstrated that the addition of iron-modified biochar can enhance denitrification. The removal of Zn²⁺ and BPA was primarily attributed to adsorption and coprecipitation mediated by biogenic precipitates formed during bioreactor operation. Through high-throughput sequencing and metabolic pathway analysis, it was found that Bacteroidia, Alphaproteobacteria, and Clostridia were key contributors to denitrification and contaminant degradation. Metabolic pathway analysis further elucidated the synergistic relationships between these microbial communities and the iron-modified biochar. This research provides new insights into the treatment of complex industrial wastewater using multifunctional microbial systems.

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铁修饰生物炭复合菌丝球生物反应器同时去除硝酸盐、锌和双酚A：优化和微生物机制

本研究利用铁修饰生物炭复合菌丝球（CMPs）固定化Fe2 +氧化反硝化菌株zogloea sp. FY-6，同时去除硝酸盐（NO3——N）、锌（Zn2+）和双酚A （BPA）。在水力停留时间（HRT）为8 h、pH为7.0、进水浓度为25.0 mg L−1 NO3——N、1.0 mg L−1 Zn2+和1.0 mg L−1 BPA的条件下，生物反应器达到最佳效果，NO3——N、Zn2+和BPA的去除率分别为87.2%、92.4%和94.4%。实验结果表明，添加铁改性生物炭可以增强脱氮效果。Zn2+和BPA的去除主要是由于生物反应器运行过程中形成的生物沉淀介导的吸附和共沉淀。通过高通量测序和代谢途径分析发现，Bacteroidia、Alphaproteobacteria和Clostridia是反硝化和污染物降解的关键贡献者。代谢途径分析进一步阐明了这些微生物群落与铁修饰生物炭之间的协同关系。本研究为利用多功能微生物系统处理复杂工业废水提供了新的见解。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.