Isolation and identification of a centromeric tandemly repeated DNA sequence in Saccharina japonica

IF 5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2026-04-01 Epub Date: 2026-03-06 DOI:10.1016/j.algal.2026.104624

Yi-Yan Cai , Cheng Zhang , Si-Qi Chen , Zhi Li , Zhi-Gang Zhou , Yan-Hui Bi

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Abstract

The centromere is a chromosomal locus essential for accurate chromosome segregation, typically composed of repetitive DNA sequences. To date, the centromeric DNA of the commercially important brown alga Saccharina japonica remains uncharacterized. In this study, we screened a candidate centromeric satellite DNA, Sjsat998, through bioinformatic analysis of male gametophyte genome data using RepeatExplorer2 and local BLAST against a centromeric BAC clone. Sjsat998 was organized as tandem repeats with a 998-bp monomer. Fluorescence in situ hybridization (FISH) confirmed its specific enrichment at centromeric regions. Immuno-FISH (IF-FISH) demonstrated precise co-localization of Sjsat998 with the centromeric histone H3 variant SjCENH3. Furthermore, electrophoretic mobility shift assays (EMSAs) confirmed the specific binding of recombinant SjCENH3 protein to Sjsat998. Sjsat998 was the first identified centromeric element in S. japonica. It provides a foundation for studying centromere evolution in brown algae and will facilitate the construction of a telomere-to-telomere (T2T) genome for this species.

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日本糖精一个着丝粒串联重复DNA序列的分离与鉴定

着丝粒是染色体精确分离所必需的染色体位点，通常由重复的DNA序列组成。迄今为止，商业上重要的褐藻Saccharina japonica的着丝粒DNA仍未被表征。在这项研究中，我们通过使用RepeatExplorer2和本地BLAST对雄性配子体基因组数据进行生物信息学分析，筛选出候选着丝粒卫星DNA Sjsat998。Sjsat998被组织为具有998-bp单体的串联重复序列。荧光原位杂交（FISH）证实了其在着丝粒区域的特异性富集。免疫- fish （IF-FISH）证实Sjsat998与着丝粒组蛋白H3变体SjCENH3精确共定位。此外，电泳迁移转移实验（EMSAs）证实了重组SjCENH3蛋白与Sjsat998的特异性结合。Sjsat998是粳稻中第一个鉴定到的着丝粒元素。这为研究褐藻着丝粒进化奠定了基础，并为该物种的端粒到端粒（T2T）基因组的构建提供了便利。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment