Agrobacterium-mediated transformation of Chlamydomonas reinhardtii to produce recombinant human serum albumin

IF 5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2026-04-01 Epub Date: 2026-03-03 DOI:10.1016/j.algal.2026.104619

Pratheesh Prakasam Thanka , Vineetha Mohan , Muraleedhara Kurup , Menatalla M. Ismail , Mallik Sudhanshu , Abdel-Hamid I. Mourad

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Abstract

Human serum albumin (HSA) constitutes the most significant fraction of proteins in human blood plasma. It remains one of the most widely used therapeutic biomolecules in modern medicine, with global demand exceeding 500 t annually. Limitations in plasma-derived HSA (pHSA), including supply shortages and the risk of pathogen transmission, have accelerated the pursuit of recombinant production strategies. This study demonstrates the first successful expression of structurally and functionally validated rHSA in Chlamydomonas reinhardtii through Agrobacterium-mediated nuclear transformation, highlighting the platform's novelty. Molecular analyses validated stable integration and transcriptional activity of the transgene, while expression levels reached up to 0.5% of total soluble protein. Structural and functional assessments confirmed that algal-derived rHSA closely resembles native pHSA, and cytotoxicity assays (MTT) verified its safety in human cell cultures. Taken together, the results highlight C. reinhardtii as an economically viable and scalable platform for sustainable biomanufacturing of high-value therapeutic proteins, making it an attractive alternative to traditional production sources.

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农杆菌介导的莱茵衣单胞菌转化制备重组人血清白蛋白

人血清白蛋白（HSA）是人血浆中最重要的蛋白质成分。它仍然是现代医学中应用最广泛的治疗性生物分子之一，全球需求量每年超过500吨。血浆源性HSA （pHSA）的局限性，包括供应短缺和病原体传播的风险，加速了对重组生产策略的追求。本研究首次通过农杆菌介导的核转化在莱茵衣藻中成功表达了结构上和功能上经过验证的rHSA，突出了该平台的新颖性。分子分析证实了转基因的稳定整合和转录活性，而表达水平高达总可溶性蛋白的0.5%。结构和功能评估证实，藻类衍生的rHSA与天然的pHSA非常相似，细胞毒性试验（MTT）证实了其在人类细胞培养中的安全性。综上所述，这些结果突出了C. reinhardtii作为经济上可行且可扩展的高价值治疗性蛋白质可持续生物制造平台，使其成为传统生产来源的有吸引力的替代品。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment