Metabolite-mediated nematicidal activity of tomato rhizobacteria against the root-knot nematode, Meloidogyne incognita

IF 3.6 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2026-05-01 Epub Date: 2026-02-25 DOI:10.1016/j.pmpp.2026.103171

Deeikshana T , Devrajan K , Prabhu S , Shandeep S G , Arun A , Thangamani C , Maruthasalam S , Poorniammal R , Haran M S R

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Abstract

The root-knot nematode, Meloidogyne incognita, poses a significant threat to global tomato production, resulting in substantial yield reductions and economic losses. The current study focused on isolating rhizosphere bacteria with nematicidal activity from major tomato growing regions in Tamil Nadu, India, and the results revealed that among isolated bacterial communities, Bacillus tequilensis isolate TRRB-2 exhibited 100% inhibition in egg hatching and juvenile mortality of M. incognita. Gas chromatography-mass spectrometry (GC-MS) analysis of B. tequilensis isolate TRRB-2 cell-free culture supernatants (CFS) revealed the presence of unique biomolecules such as 5,6-decanediol, E−15-heptadecenal, 1,2-benzenedicarboxylic acid, dimethyl palmitamine, 2′,4′-dihydroxyacetophenone, 4,7-ethanoisobenzofuran, and 1,2-benzenediol. The results of molecular docking studies exhibited that among the different biomolecules, 5,6-decanediol demonstrated strong binding affinity against Vitellogenin, SXP/RAL-2, ODR-3, Ferritin, Acetylcholinesterase, and Protein Disulfide Isomerase (PDI) targets of M. incognita. Furthermore, B. tequilensis isolate TRRB-2 exhibited a 75.35% reduction in nematode populations under greenhouse conditions with an 82% increase in overall plant growth parameters. The results of the field trials revealed that soil drenching of B. tequilensis isolate TRRB-2 resulted in a 70% reduction in soil and root population of M. incognita, along with a 60% increase in overall plant growth parameters when compared to the control. Overall, the current study highlights the importance of the rhizosphere-isolated B. tequilensis isolate TRRB-2 for the successful management of M. incognita in tomato.

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代谢物介导的番茄根杆菌对根结线虫的杀线虫活性研究

根结线虫（Meloidogyne incognita）对全球番茄生产构成重大威胁，导致产量大幅下降和经济损失。本研究从印度泰米尔纳德邦主要番茄产区分离出具有杀线虫活性的根际细菌，结果表明，在分离出的细菌群落中，龙舌兰芽孢杆菌TRRB-2具有100%的卵孵化抑制作用，并能抑制不认识小蠊的幼虫死亡率。气相色谱-质谱联用（GC-MS）分析了龙酒芽孢杆菌TRRB-2无细胞培养上清（CFS），发现了5,6-癸二醇、E−15-十七烯醛、1,2-苯二甲酸、二甲基棕榈胺、2 ',4 ' -二羟基苯乙酮、4,7-乙醇异苯呋喃和1,2-苯二醇等独特的生物分子。分子对接研究结果表明，在不同的生物分子中，5,6-十烷二醇对M. incognita的卵黄原蛋白、SXP/RAL-2、ODR-3、铁蛋白、乙酰胆碱酯酶和蛋白二硫异构酶（PDI）靶点具有较强的结合亲和力。此外，在温室条件下，龙舌兰芽孢杆菌TRRB-2的线虫种群数量减少了75.35%，植物整体生长参数增加了82%。田间试验结果表明，与对照相比，龙舌兰芽孢杆菌TRRB-2对土壤进行淋雨处理后，黑穗兰土壤和根系种群数量减少70%，植株整体生长参数增加60%。综上所述，本研究强调了龙舌兰芽孢杆菌根际分离株TRRB-2对于成功管理番茄中隐匿性黑僵菌的重要性。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.