Interactions among progesterone, estradiol, and γ-aminobutyric acid in rat spermatozoal hyperactivation.

IF 2.1 4区生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-03-06 DOI:10.1262/jrd.2025-106

Miki Muroi, Shiho Fukuda, Miki Takahashi, Tatsuya Suzuki, Masakatsu Fujinoki

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引用次数: 0

Abstract

Our previous study in hamsters indicated that progesterone (P) induces enhancement of spermatozoal hyperactivation, while 17β-estradiol (Eβ) and γ-aminobutyric acid (GABA) suppress this enhancement. Since P has also been shown to enhance hyperactivation of rat spermatozoa in another study conducted by our team, we examined the effects of Eβ and GABA on the P-enhanced hyperactivation of rat spermatozoa in the present study. Our results showed that Eβ suppressed P-enhanced hyperactivation in a dose-dependent manner through an estrogen receptor. In contrast, GABA did not affect P-enhanced hyperactivation. Instead, 5-500 fM GABA significantly enhanced spermatozoal hyperactivation, and this GABA-induced enhancement of hyperactivation was associated with a GABA_A receptor. In conclusion, P-enhanced hyperactivation of rat spermatozoa was suppressed by Eβ although it was not suppressed by GABA. Moreover, low concentrations of GABA enhanced rat spermatozoal hyperactivation.

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孕酮、雌二醇和γ-氨基丁酸在大鼠精子过度活化中的相互作用。

我们之前在仓鼠的研究表明，孕酮(P)诱导精子过度活化的增强，而17β-雌二醇（Eβ）和γ-氨基丁酸（GABA）抑制这种增强。由于在我们团队的另一项研究中P也被证明可以增强大鼠精子的过度激活，因此我们在本研究中检测了Eβ和GABA对P增强大鼠精子过度激活的影响。我们的研究结果表明，Eβ通过雌激素受体以剂量依赖的方式抑制p增强的过度激活。相比之下，GABA不影响p增强的过度激活。相反，5-500 fM GABA显著增强了精子的过度激活，而这种GABA诱导的过度激活的增强与GABAA受体有关。综上所述，β β可以抑制p增强的大鼠精子超激活，而GABA则不具有抑制作用。此外，低浓度的GABA增强了大鼠精子的过度激活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Reproduction and Development 生物-奶制品与动物科学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

2 months

期刊介绍： Journal of Reproduction and Development (JRD) is the official journal of the Society for Reproduction and Development, published bimonthly, and welcomes original articles. JRD provides free full-text access of all the published articles on the web. The functions of the journal are managed by Editorial Board Members, such as the Editor-in-Chief, Co-Editor-inChief, Managing Editors and Editors. All manuscripts are peer-reviewed critically by two or more reviewers. Acceptance is based on scientific content and presentation of the materials. The Editors select reviewers and correspond with authors. Final decisions about acceptance or rejection of manuscripts are made by the Editor-in-Chief and Co-Editor-in-Chief.