L-2-aminoadipic acid inhibits porcine epidemic diarrhea virus replication by targeting and reducing cellular autophagy

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2026-05-01 Epub Date: 2026-03-03 DOI:10.1016/j.vetmic.2026.110967

Xiao Ma , Hongbo Cui , Runze Song , Yanfei Huang , Yilei Li , Junxing Ma , Hongying Chen , Shijie Ma , Zhanyong Wei

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引用次数: 0

Abstract

Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) is an acute and highly contagious intestinal infectious disease. The metabolic alterations during PEDV infection remain largely unexplored. In this study, LLC-PK1 cells infected with PEDV were subjected to non-targeted metabolomics analysis. Notably, the amino acid metabolite L-2-aminoadipic acid (L-2AA) was significantly upregulated, while indole-3-acetic acid (3-IAA) exhibited a marked downregulation following PEDV infection. Subsequent investigations revealed that both L-2AA and 3-IAA significantly hindered the late stage of viral propagation. Mechanistically, aldehyde dehydrogenase 7A1 (ALDH7A1), a key enzyme involved in the biosynthesis of L-2AA, mediates the PEDV-induced upregulation of L-2AA and functions to negatively regulate viral replication. Detailed analysis indicated that L-2AA mitigates PEDV infection via reducing autophagic activity. These data support a novel mechanism used by L-2AA to downregulate autophagic activity to block viral replication and provide potential anti-PEDV drug targets.

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l -2-氨基己二酸通过靶向和减少细胞自噬抑制猪流行性腹泻病毒复制。

由猪流行性腹泻病毒（PEDV）引起的猪流行性腹泻是一种急性、高传染性的肠道传染病。PEDV感染期间的代谢改变在很大程度上仍未被探索。在本研究中，对感染PEDV的LLC-PK1细胞进行了非靶向代谢组学分析。值得注意的是，感染PEDV后，氨基酸代谢物l -2-氨基己二酸（L-2AA）显著上调，而吲哚-3-乙酸（3-IAA）显著下调。随后的研究发现，L-2AA和3-IAA都显著阻碍了病毒的后期繁殖。从机制上说，醛脱氢酶7A1 （ALDH7A1）是参与L-2AA生物合成的关键酶，可介导pedv诱导的L-2AA上调，并发挥负调控病毒复制的作用。详细分析表明，L-2AA通过降低自噬活性来减轻PEDV感染。这些数据支持了L-2AA下调自噬活性以阻断病毒复制的新机制，并提供了潜在的抗pedv药物靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.