Metagenomics reveals an interaction among rhizosphere microbial community, soil properties and active ingredients in a medicinal crop Panax notoginseng

IF 7.5 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research Pub Date : 2026-03-01 Epub Date: 2025-11-21 DOI:10.1016/j.jgr.2025.11.006

Zheng-Wei Liang , Yan-Hui Guan , Zheng Lv , Sheng-Chao Yang , Ming Zhao , Jun-Wen Chen

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引用次数: 0

Abstract

Background

This study aimed to examine the effects of intensive cultivation practices on the rhizosphere microecology of Panax notoginseng. Additionally, we sought to compare these practices with an understory cultivation model that was intended to mimic native growth conditions, with the objective of improving the quality of Radix Notoginseng.

Methods

The total saponin and active ingredient content in both cultivation methods were quantified using high-performance liquid chromatography (HPLC). The nutrients of the rhizosphere soils associated with both cultivation methods (understory cultivated P. notoginseng rhizosphere soil [UCPS] and intensive cultivated P. notoginseng rhizosphere soil [ICPS]) were analyzed. The microbial communities present in UCPS and ICPS were characterized using metagenomic sequencing.

Results

The underground biomass accounted for 71.21 % and 74.00 % of the total biomass in understory cultivated P. notoginseng (UCPn) and intensively cultivated P. notoginseng (ICPn), respectively. The total saponin content in the main root of UCPn was found to be 109.24 ± 3.40 mg/g, compared to 91.31 ± 5.82 mg/g in ICPn. The concentration of medicinal ingredients (ginsenoside Rb1 + ginsenoside Rg1 + notoginsenoside R1) in UCPn was 10.83 %, while ICPn exhibited a higher concentration of 13.39 %. Microbial biomarkers identified in UCPS include Bradyrhizobium, Pseudomonas, and Paraburkholderia, which are associated with nitrogen cycling processes. In contrast, Variovorax and Sphingobium were predominant in ICPS, contributing to phosphorus metabolism.

Conclusion

Rhizosphere soil microbial biomarkers influence soil carbon nutrition, which directly impacts the quality of UCPn. The quality of ICPn is primarily determined by phosphorus-related biomarkers, with indirect influences from carbon and nitrogen nutrition.

Abstract Image

查看原文本刊更多论文

宏基因组学揭示了药用作物三七根际微生物群落、土壤性质和有效成分之间的相互作用。

背景：本研究旨在探讨集约栽培对三七根际微生态的影响。此外，我们试图将这些做法与旨在模拟本地生长条件的林下栽培模式进行比较，目的是提高三七的质量。方法：采用高效液相色谱法对两种栽培方法中总皂苷和有效成分含量进行定量分析。对两种栽培方式（林下栽培三七根际土壤[UCPS]和集约栽培三七根际土壤[ICPS]）的根际土壤养分进行了分析。利用宏基因组测序对UCPS和ICPS中存在的微生物群落进行了表征。结果：林下栽培三七（UCPn）和集约栽培三七（ICPn）地下生物量占总生物量的71.21%和74.00%。UCPn主根总皂苷含量为109.24±3.40 mg/g， ICPn为91.31±5.82 mg/g。UCPn中药用成分（人参皂苷Rb1 +人参皂苷Rg1 +三七皂苷R1）的浓度为10.83%，而ICPn的浓度更高，为13.39%。在UCPS中发现的微生物生物标志物包括与氮循环过程相关的慢生根瘤菌、假单胞菌和副aburkholderia。相比之下，在ICPS中，Variovorax和Sphingobium占优势，有助于磷的代谢。结论：根际土壤微生物生物标志物影响土壤碳营养，直接影响UCPn质量。ICPn的质量主要由磷相关的生物标志物决定，碳和氮营养也有间接影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE

CiteScore

11.40

自引率

9.50%

发文量

111

审稿时长

6-12 weeks

期刊介绍： Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.