Efficacy of Calcium Silicate-Based, Simvastatin, Levofloxacin and Calcium Hydroxide Intracanal Medicaments Against Mature Enterococcus faecalis Biofilm. An In Vitro Study

IF 1.7 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2026-08-04 Epub Date: 2026-03-04 DOI:10.1111/aej.70070

Nadia Saeed Hosny, Sara Gamal Elgamal

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Abstract

This in vitro study investigated the antibiofilm efficacy of calcium silicate-based, Simvastatin, Levofloxacin and calcium hydroxide intracanal medicaments against mature Enterococcus faecalis biofilm. Ninety dentine specimens were inoculated with Enterococcus faecalis biofilm for three weeks and divided into five groups (n = 18): (1) calcium silicate-based; (2) Simvastatin; (3) Levofloxacin; (4) calcium hydroxide (CH); (5) positive control, no medicament. After two weeks of intracanal medicament application, live/dead bacterial cells were assessed using confocal laser scanning microscopy. The results revealed a statistically significant difference in the percentage of dead bacteria of the four tested groups compared to the positive control group (H = 30.45, p < 0.001). The calcium silicate-based group recorded the highest median (IQR) percentage of dead bacteria at 56.33 (10.03), followed by Simvastatin 44.88 (7.30), CH 41.70 (12.53), Levofloxacin 35.99 (3.78), and the positive control group 3.61 (1.01). These findings suggest that these intracanal medicaments demonstrate promising antibiofilm activity, with further investigations needed.

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硅酸钙基、辛伐他汀、左氧氟沙星和氢氧化钙肛管内药物对成熟粪肠球菌生物膜的作用。一项体外研究

本实验研究了硅酸钙基、辛伐他汀、左氧氟沙星和氢氧化钙四种肛管内药物对成熟粪肠球菌生物膜的抗菌效果。90份牙本质标本接种粪肠球菌生物膜3周后，分为5组（n = 18）：(1)硅酸钙组；(2)辛伐他汀;(3)左氧氟沙星;(4)氢氧化钙（CH）；(5)阳性对照，不用药。两周后，使用共聚焦激光扫描显微镜评估活/死细菌细胞。结果显示，与阳性对照组相比，4个试验组的死菌百分比有统计学差异(H = 30.45, p

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.