Evaluation of demineralization changes utilizing the Canary Caries Detection System and various toothpastes on incipient enamel lesions.

Abstract

Purpose: The Canary Caries Detection System has been shown to quantify the degree of demineralization of incipient enamel. The objective of this study was to evaluate and compare demineralization changes after applying various toothpastes on incipient enamel lesions utilizing the Canary Caries Detection System.

Materials and methods: Thirty-five extracted human teeth premolars were divided into seven groups (n=5). Each group was demineralized in a lactate acid buffer solution (pH 4.5) and tested using one of six toothpastes or a phosphate buffer solution (control, pH 7.4). The teeth were brushed according to manufacturer's instructions daily for 28 days. Canary Numbers, standardized cold light, and fluorescent photographs were collected at the initial (T0), following demineralization (T1), day 14 (T2), and day 28 (T3).

Results: It was found that there was a statistical difference between all groups and the control between T2-T3 (p<0.046 facial surfaces) and T1-T3 (p<0.015 lingual surfaces). There was also a statistical difference between T1-T3 when comparing all groups. The cold light and fluorescent light photos also show evidence of changes in demineralization from T1-T3 in the PreviDent 5000 Plus group.

Conclusion: The PreviDent 5000 Plus group demonstrated a statistical difference to Clinpro 5000, MI Paste Plus and PreviDent 5000 Booster Plus from T1-T3 as the Canary Numbers decreased. There was an increase and significant difference in the PBS group Canary Numbers from T1-T3, indicating the negative control showed continued demineralization.