Comparative study of the effects of subcutaneously administered umbilical cord-derived mesenchymal stem cells and exosomes derivatives on wound healing in a chemical burn model of alkaline-induced skin burns.
Hamit Hakan Bekir, Mehmet Gurdal, Ozlem Barut Selver, Banu Yaman, Cansu Subasi Demir, Meltem Kocamanoglu, Ayberk Sahin, Ahmet Bicer
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引用次数: 0
Abstract
Background: In burn-injured skin, the main goal is to accelerate wound healing and restore the barrier. In chemical burns, excessive inflammation and deep tissue damage complicate repair. Regenerative medicine approaches may modulate these processes. This study investigated the effects of human umbilical cord-derived mesenchymal stem cells (HucMSCs) and their exosomes (Exo-HucMSCs) on wound healing in a chemical burn model.
Methods: A chemical burn was induced on the dorsal skin of 24 Wistar rats using 2 M sodium hydroxide. Rats were randomly assigned to three groups (n = 8): HucMSC-treated, Exo-HucMSC-treated and control. Wound closure and histopathological changes were evaluated and compared among groups.
Results: Wound closure was higher in the HucMSC group (78.15% ± 9.19) than in the Exo-HucMSC (66.11% ± 7.33) and control groups (57.35% ± 4.54) (p < 0.05). Histologically, treatment groups showed early inflammatory changes on days 1 and 3 (p < 0.01), enhanced striated muscle regeneration on day 7 (p < 0.01) and narrower non-epithelialized areas on day 21 (p < 0.05). Neoangiogenesis was greater in the Exo-HucMSC group on day 3 (p < 0.01).
Conclusion: Both HucMSC and Exo-HucMSC treatment accelerated chemical burn healing. Exosomes promoted early-phase angiogenesis, whereas HucMSCs provided superior long-term wound closure.
背景：在烧伤损伤的皮肤中，主要目标是加速伤口愈合和恢复屏障。在化学烧伤中，过度的炎症和深层组织损伤使修复复杂化。再生医学方法可以调节这些过程。本研究探讨了人脐带源性间充质干细胞（HucMSCs）及其外泌体（Exo-HucMSCs）对化学烧伤模型创面愈合的影响。方法：用2 M氢氧化钠诱导24只Wistar大鼠背部皮肤化学烧伤。大鼠随机分为三组（n = 8）： hucmsc治疗组、exo - hucmsc治疗组和对照组。比较各组创面闭合情况和组织病理学变化。结果：HucMSC组创面愈合率（78.15%±9.19）高于Exo-HucMSC组（66.11%±7.33）和对照组（57.35%±4.54）（p p p p p）。结论：HucMSC和Exo-HucMSC治疗均能促进化学烧伤愈合。外泌体促进早期血管生成，而HucMSCs提供优越的长期伤口愈合。
期刊介绍：
Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization.
Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community.
Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.
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