Mesenchymal stem/stromal cell secretomes generated in serum free conditions and on clinically relevant plasma polymerized membranes promote fibroblast wound healing activity.

IF 3 4区医学 Q4 CELL & TISSUE ENGINEERING

Regenerative medicine Pub Date : 2026-02-01 Epub Date: 2026-03-05 DOI:10.1080/17460751.2026.2638902

Harish Deepak Ghaisas, Jide Tope Olanipekun, Karthik Vaidya, Jees Thomas, Shilpy Dantuluri, Tejaswi Naradasu, Ruth Taylor, David Haddow, Vesna Stanulovic, Maarten Hoogenkamp, Douglas Ward, Geoffrey Brown, Charlotte H Hulme, Karina T Wright, Hideaki Nakajima, William Eustace Basil Johnson

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引用次数: 0

Abstract

Background/aims: Mesenchymal stem/stromal cells (MSCs) are known to secrete wound healing factors. However, the delivery of the MSCs and their secretomes remains a barrier to the development of a successful regenerative medicine. Therefore, we examined the content and paracrine activity of serum free MSC conditioned media (MSC CM) generated in routine tissue culture and when cultured on a plasma polymerized membrane previously used clinically in cell therapies for skin wounds.

Methods: Serum free MSC CM from murine and human MSC cultures were subjected to proteomic analysis by mass spectrometry and quantitative immunoassays and their paracrine effects were tested on dermal fibroblasts in vitro.

Results: MSC CM contained multiple wound healing factors, including extracellular matrix proteins and soluble factors. MSC CM harvested from flask cultures and plasma polymerized membrane cultures significantly increased dermal fibroblast adhesion, proliferation, and scratch wound closure (for mouse MSC CM) compared to control media.

Conclusion: This study has: (i) shown that MSC secreted factors increased dermal fibroblast activities seen in wound healing; (ii) identified target factors potentially responsible for these paracrine effects; (iii) shown that MSC secretomes generated on a plasma polymerized membrane used in skin cell therapies also have wound healing paracrine effects.

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无血清条件下和临床相关的血浆聚合膜上产生的间充质干细胞/基质细胞分泌组促进成纤维细胞伤口愈合活性。

背景/目的：间充质干细胞（MSCs）可以分泌伤口愈合因子。然而，间充质干细胞及其分泌体的递送仍然是成功发展再生医学的障碍。因此，我们检测了在常规组织培养中产生的无血清间充质干细胞条件培养基（MSC CM）的含量和旁分泌活性，以及在先前临床用于皮肤伤口细胞治疗的血浆聚合膜上培养时的含量和旁分泌活性。方法：采用质谱法和定量免疫分析法对小鼠和人间充质干细胞培养的无血清间充质干细胞进行蛋白质组学分析，并检测其对体外真皮成纤维细胞的旁分泌作用。结果：MSC CM含有多种伤口愈合因子，包括细胞外基质蛋白和可溶性因子。与对照培养基相比，从烧瓶培养和血浆聚合膜培养中收获的MSC CM显著增加了真皮成纤维细胞的粘附、增殖和划伤愈合（小鼠MSC CM）。结论：本研究：(1)表明间充质干细胞分泌因子增加了创面愈合过程中真皮成纤维细胞的活性；（ii）确定可能导致这些旁分泌效应的目标因素；（iii）表明在用于皮肤细胞治疗的血浆聚合膜上产生的间充质干细胞分泌组也具有伤口愈合的旁分泌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.