{"title":"Promoter Sequences Do Not Solely Govern nosZ Expression Differences between Bradyrhizobium ottawaense and B. diazoefficiens.","authors":"Sawa Wasai-Hara, Yoshikazu Shimoda, Hisayuki Mitsui, Shusei Sato, Haruko Imaizumi-Anraku, Kiwamu Minamisawa","doi":"10.1264/jsme2.ME25079","DOIUrl":null,"url":null,"abstract":"<p><p>Nitrous oxide (N<sub>2</sub>O) is a potent greenhouse gas, and the enzyme Nos catalyzes its reduction to dinitrogen (N<sub>2</sub>). Bradyrhizobium ottawaense exhibits strong N<sub>2</sub>O-reducing activity with high nosZ expression. To investigate whether promoter sequences affect nosZ expression, we constructed reciprocal promoter-swapped mutants between B. ottawaense and B. diazoefficiens. The swapping of promoters did not significantly affect expression levels. B. ottawaense mutants maintained approximately 200-fold higher expression levels than B. diazoefficiens, and the introduction of the B. ottawaense promoter into B. diazoefficiens did not increase expression levels. Therefore, the present results indicate that promoter sequence differences are not the primary factor affecting nosZ expression, suggesting regulation by other factors.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"41 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999732/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbes and Environments","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1264/jsme2.ME25079","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Nitrous oxide (N2O) is a potent greenhouse gas, and the enzyme Nos catalyzes its reduction to dinitrogen (N2). Bradyrhizobium ottawaense exhibits strong N2O-reducing activity with high nosZ expression. To investigate whether promoter sequences affect nosZ expression, we constructed reciprocal promoter-swapped mutants between B. ottawaense and B. diazoefficiens. The swapping of promoters did not significantly affect expression levels. B. ottawaense mutants maintained approximately 200-fold higher expression levels than B. diazoefficiens, and the introduction of the B. ottawaense promoter into B. diazoefficiens did not increase expression levels. Therefore, the present results indicate that promoter sequence differences are not the primary factor affecting nosZ expression, suggesting regulation by other factors.
期刊介绍：
Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.
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