Systematic review and meta-analysis of fertility outcome following in vivo insemination with sex-sorted semen in sheep.

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Journal of the South African Veterinary Association Pub Date : 2025-11-01 DOI:10.36303/JSAVA.696

J G Nel, P N Thompson, D E Holm

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引用次数: 0

Abstract

Background: Sex-sorted semen (SS) offers economic benefits to sheep producers, but lower fertility outcome (FO), defined as probability of pregnancy, pregnancy rate or lambing rate, than that following conventional semen (CS) artificial insemination (AI) may limit its use.

Objectives: To systematically review the literature, and analyse factors associated with FO following AI in sheep using SS vs CS.

Methods: Available literature was searched using the PRISMA guidelines, resulting in 11 studies with 14 experiments that were reviewed. From these, information from 70 study cohorts representing 13 experiments was used to conduct a meta-analysis which confirmed that FOs for SS and CS AI were 37% (95% CI = 31-43%) and 52% (95% CI = 45-59%), respectively (p < 0.01).

Results: FO improved over time, with FO following SS and CS AI improving at the same rate over the period investigated (1997- 2022). In a subgroup analysis, five factors were identified that potentially decrease the FO after SS AI disproportionately. These were sperm dose ≤ 4 × 10⁶ spermatozoa, semen preservation (fresh vs frozen-thawed), oestrus synchronisation using an intravaginal sponge (vs a controlled internal drug release device), presence of gonadotropin-releasing hormone in the synchronisation protocol, and absence of biostimulation using a teaser ram. In a random-effects model analysis, an interaction between sperm dose and SS vs CS, and semen preservation were independent predictors of FO after adjusting for the effect of timing of insemination.

Conclusion: FO following insemination with SS is 15% lower than that of CS, which can be narrowed by increasing the semen dose for SS inseminations and improving the synchronisation of ovulation with the timing of insemination.

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绵羊体内用性别分类精液授精后生育结果的系统评价和荟萃分析。

背景：性别分类精液（SS）为绵羊养殖者提供了经济效益，但与常规精液（CS）人工授精（AI）相比，较低的生育结局（FO）（定义为妊娠概率、妊娠率或产羔率）可能限制其使用。目的：系统地回顾文献，并利用SS和CS分析绵羊AI后FO的相关因素。方法：按照PRISMA指南检索现有文献，对11项研究、14项实验进行综述。从中，来自70个研究队列代表13个实验的信息被用于进行荟萃分析，证实SS和CS AI的FOs分别为37% （95% CI = 31-43%）和52% (95% CI = 45-59%) （p < 0.01）。结果：FO随着时间的推移而改善，FO紧随SS和CS AI在调查期间（1997- 2022）以相同的速度改善。在亚组分析中，确定了五个因素，这些因素可能会不成比例地降低SS AI后的FO。这些试验包括精子剂量≤4 × 106个精子，精液保存（新鲜与冻融），使用阴道内海绵（与受控的内部药物释放装置）进行发情同步，在同步方案中使用促性腺激素释放激素，以及使用刺激雄羊进行生物刺激。在随机效应模型分析中，在调整了授精时间的影响后，精子剂量与SS / CS和精液保存之间的相互作用是FO的独立预测因子。结论：SS人工授精后FO比CS低15%，可通过增加SS人工授精的精液剂量和提高排卵与授精时间的同步性来缩小FO。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the South African Veterinary Association VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： The Journal of the South African Veterinary Association is a contemporary multi-disciplinary scientific mouthpiece for Veterinary Science in South Africa and abroad. It provides veterinarians in South Africa and elsewhere in the world with current scientific information across the full spectrum of veterinary science. Its content therefore includes reviews on various topics, clinical and non-clinical articles, research articles and short communications as well as case reports and letters.