Quantification of Angora goat beta chain haemoglobin variants using the Sebia Capillarys 2 Flex-Piercing system.

IF 0.5 4区 农林科学 Q3 VETERINARY SCIENCES
S D C Parsons, A Vermeulen, M Hobson, E H Hooijberg
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引用次数: 0

Abstract

This study aimed to evaluate the utility of the Sebia Capillarys 2 Flex-Piercing (C2FP) system for the quantification of the major caprine beta chain haemoglobin (Hb) variants, i.e. foetal Hb, neonatal Hb, and adult Hb. Blood samples were collected from six Angora kids at one, 11, and 20 weeks after birth, and analysed using the C2FP instrument and Capillarys Hemoglobin(E) kit. Electropherograms displayed three major peaks which showed consistent changes in relative magnitude as kids aged. At one week old, a peak at position ~75 was identified as foetal Hb, while in older animals, peaks at ~150 and ~170 were identified as neonatal Hb and adult Hb, respectively. In conclusion, this study has confirmed the utility of the Sebia C2FP system for the simple, rapid, and standardised quantification of the major beta chain Hb variants of Angora goats.

使用Sebia capillys 2柔性穿刺系统定量安哥拉山羊β链血红蛋白变异。
本研究旨在评估Sebia Capillarys 2 flex -穿刺(C2FP)系统对主要山羊β链血红蛋白(Hb)变异(即胎儿Hb、新生儿Hb和成人Hb)的定量应用。在出生后1周、11周和20周采集了6名安哥拉儿童的血液样本,并使用C2FP仪器和毛细管血红蛋白(E)试剂盒进行分析。随着儿童年龄的增长,电图显示出三个主要的峰,它们的相对大小变化一致。在1周龄时,~75处的峰值被确定为胎儿Hb,而在老年动物中,~150和~170处的峰值分别被确定为新生儿Hb和成人Hb。总之,本研究证实了Sebia C2FP系统用于安哥拉山羊主要β链Hb变异的简单、快速和标准化定量的实用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.50
自引率
0.00%
发文量
23
审稿时长
22 weeks
期刊介绍: The Journal of the South African Veterinary Association is a contemporary multi-disciplinary scientific mouthpiece for Veterinary Science in South Africa and abroad. It provides veterinarians in South Africa and elsewhere in the world with current scientific information across the full spectrum of veterinary science. Its content therefore includes reviews on various topics, clinical and non-clinical articles, research articles and short communications as well as case reports and letters.
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