Titus Tugume, Bernard Erima, Graham A Matulis, Hannah Kibuuka, Ambrose K Musinguzi, Joseph Asea, James Kiyengo, Edison Mworozi, William R Dorman, Hadish H Yohannes, Haley P Smith, Korey L Delp, Fred Wabwire-Mangen, Timothy E Egbo, Eric Garges, Michael von Fricken, Christopher P Stefan, Jeffrey W Koehler, Denis K Byarugaba
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引用次数: 0
Abstract
Dengue virus (DENV) is widely distributed across Africa but underreported due to limited surveillance and diagnostic capacity. Under an acute febrile illness surveillance protocol, we collected and screened 374 samples from patients with fever in Mogadishu, Somalia. Samples were screened for DENV serotypes using real-time reverse transcription polymerase chain reaction, and positive samples were sequenced and phylogenetically analyzed. We identified 55 samples containing orthoflavivirus RNA (14.7%). Of these 55 samples, three dengue serotypes, DENV serotype 1 (DENV-1, n = 24), DENV serotype 2 (DENV-2, n = 21), and DENV serotype 3 (DENV-3, n = 12), were identified. Two cases of coinfections were detected: 1) between DENV-1 and DENV-2 and 2) between DENV-2 and DENV-3. DENV-1 had two genetic clusters, both related to lineages from Asia. DENV-2 grouped within a set of sequences from Asia and Africa, particularly close to a DENV-2 outbreak in Kenya. The DENV-3 grouped with isolates from Oceania Asia, clustering with a case of dengue fever identified in Italy in 2023 and Ethiopia in 2023. Our findings suggest concurrent infections of the three serotypes with DENV-3 being more widely spread in East Africa.
登革热病毒（DENV）在非洲广泛分布，但由于监测和诊断能力有限而报告不足。根据急性发热性疾病监测方案，我们从索马里摩加迪沙的发热患者中收集并筛选了374份样本。采用实时逆转录聚合酶链反应对样本进行DENV血清型筛选，对阳性样本进行测序和系统发育分析。我们鉴定出55份含有正黄病毒RNA的样本（14.7%）。在这55份样本中，鉴定出3种登革热血清型，即DENV血清1型（DENV-1, n = 24）、DENV血清2型（DENV-2, n = 21）和DENV血清3型（DENV-3, n = 12）。共感染2例：1)DENV-1与DENV-2、2)DENV-2与DENV-3。DENV-1有两个遗传集群，都与来自亚洲的谱系有关。DENV-2属于来自亚洲和非洲的一组序列，特别是在肯尼亚DENV-2暴发附近。DENV-3与来自大洋洲和亚洲的分离株归为一类，与2023年在意大利和2023年在埃塞俄比亚发现的一例登革热病例聚集在一起。我们的研究结果表明，三种血清型的同时感染与DENV-3在东非更为广泛地传播。
期刊介绍：
The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine.
The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development.
The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal.
Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries
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