Response Mechanism of Lactobacillus plantarum under Simulated Digestion Based on Proteomics and Metabolomics Analysis

IF 1.1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2025-12-23 DOI:10.1134/S0003683825600034

L. Zhao, Z. Cheng, Y. Ling, Y. Wu, M. Zhang, H. Yang, Z. Li

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Abstract

Proteomics and a metabolomics strategy were used to investigate the probiotic properties of Lactobacillus plantarum BW2013 under simulated in vitro digestion. Thirty seven significant differential proteins including 6-phosphogluconate dehydrogenase, pyruvate kinase, F0F1 ATP synthase, ABC-type transporters, ribosomal and universal stress proteins and 40 significant differential metabolites, such as indole-3-lactic acid, Pro, trans-cinnamic acid, betaine, citric acid, D-fructose, and γ-aminobutyric acid were identified and characterized. The association analysis indicated that most upregulated proteins and metabolites are involved primarily in stress response, regulation of intestinal flora and accumulation of beneficial substances relating to carbohydrate and energy metabolic pathways, amino acid metabolism pathways, nucleotide metabolism pathways, overall/universal stress response. The results provide new insights into the response mechanism of L. plantarum BW2013 under the simulated digestion conditions, facilitating the exploitation of its potential application in probiotic products.

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基于蛋白质组学和代谢组学分析的植物乳杆菌模拟消化反应机制

采用蛋白质组学和代谢组学方法研究植物乳杆菌BW2013在体外模拟消化条件下的益生菌特性。鉴定鉴定了37种显著差异蛋白，包括6-磷酸葡萄糖酸脱氢酶、丙酮酸激酶、F0F1 ATP合酶、abc型转运蛋白、核糖体和通用应激蛋白，以及40种显著差异代谢物，如吲哚-3-乳酸、Pro、反式肉桂酸、甜菜碱、柠檬酸、d -果糖和γ-氨基丁酸。关联分析表明，大多数上调蛋白和代谢物主要参与应激反应、肠道菌群的调节和有益物质的积累，这些有益物质与碳水化合物和能量代谢途径、氨基酸代谢途径、核苷酸代谢途径、整体/普遍应激反应有关。研究结果为植物乳杆菌BW2013在模拟消化条件下的响应机制提供了新的认识，为开发其在益生菌产品中的潜在应用提供了便利。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.