Calcium supply alters aquaporin transcription and enhances water deficit resilience in a Eucalyptus hybrid

IF 2.3 3区农林科学 Q2 FORESTRY

Trees Pub Date : 2026-03-02 DOI:10.1007/s00468-025-02712-z

Patricia Ramalho de Barros, Samuel Vasconcelos Valadares, Alice Pita-Barbosa, Wagner L. Araújo, Nairam Félix de Barros

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引用次数: 0

Key message

Calcium plays a role in modulating aquaporin expression, reducing oxidative stress, preserving chlorophyll content, and maintaining membrane integrity, suggesting it may enhance eucalypt resilience to water restriction.

Abstract

Drought is a significant abiotic stress that severely impacts the growth and productivity of forests worldwide. This study investigates the role of calcium (Ca²⁺) supply in modulating aquaporin (AQP) gene expression and mitigating oxidative stress in eucalypt (Eucalyptus urophylla × Eucalyptus camaldulensis) under water restriction. Three-month-old eucalypt seedlings were subjected to water restriction, with or without Ca²⁺ supplementation. We measured mRNA expression levels of five aquaporin isoforms (PIP1;2, PIP1;3, PIP2;1, PIP2;2, and PIP2;7), chlorophyll content, and markers of oxidative stress [e.g., hydrogen peroxide (H₂O₂), malondialdehyde (MDA), and the activities of catalase (CAT) and superoxide dismutase (SOD)]. Our findings reveal that initial water restriction upregulated the expression of PIP1;2, PIP2;1, PIP2;2, and PIP2;7 in roots, which likely facilitated water uptake and maintained cellular hydration. However, under prolonged water limitation, plants supplemented with Ca²⁺ showed downregulation of these PIPs, possibly as a protective mechanism to reduce water loss. In contrast, plants without Ca²⁺ exhibited an upregulation of PIP1;2, PIP1;3, and PIP2;7 under prolonged water deficit. Ca²⁺ supplementation also played a crucial role in preserving chlorophyll content and reducing oxidative damage under water limitation. Plants supplemented with Ca²⁺ had lower levels of H₂O₂ and MDA, reduced oxidative damage and membrane permeability, alongside increased CAT activity, which suggests an enhanced oxidative response. Our results provide new insights into how Ca²⁺ enhances eucalypt responses to water deprivation, enhancing our understanding of the function of Ca²⁺ in drought responses.

查看原文本刊更多论文

钙的供应改变了水通道蛋白的转录，增强了桉树杂交种的水分亏缺恢复能力

关键信息：钙在调节水通道蛋白表达、减少氧化应激、保持叶绿素含量和维持膜完整性方面发挥作用，表明它可能增强桉树对水分限制的弹性。摘要干旱是严重影响森林生长和生产力的重要非生物胁迫。本研究探讨了限水条件下钙（Ca2+）供应对尾桉（Eucalyptus urophylla × Eucalyptus camaldulensis）水通道蛋白（AQP）基因表达和氧化应激的调节作用。三个月大的桉树幼苗受到水分限制，有或没有补充Ca2+。我们测量了五种水通道蛋白异构体（PIP1、2、PIP1、3、PIP2、1、PIP2、2、PIP2、7）的mRNA表达水平、叶绿素含量和氧化应激标志物（如过氧化氢（H2O2）、丙二醛（MDA）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性）。我们的研究结果表明，初始水分限制上调了PIP1的表达；2、PIP2;1、PIP2;2、PIP2；这可能促进了水分的吸收并维持了细胞的水合作用。然而，在长时间的水分限制下，补充Ca2+的植物表现出这些pip的下调，这可能是减少水分损失的保护机制。相比之下，没有Ca2+的植物表现出PIP1的上调；2、PIP1;3、PIP2；7 .长期缺水。在水分限制条件下，补充Ca2+对保持叶绿素含量和减少氧化损伤也起着至关重要的作用。添加Ca2+的植株H2O2和MDA水平降低，氧化损伤和膜通透性降低，CAT活性增加，表明氧化反应增强。我们的研究结果为Ca2+如何增强桉树对缺水的反应提供了新的见解，增强了我们对Ca2+在干旱反应中的功能的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trees 农林科学-林学

CiteScore

4.50

自引率

4.30%

发文量

113

审稿时长

3.8 months

期刊介绍： Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.