D. S. Vinogradova, Z. A. Spiridonova, T. A. Antysheva, Zh. Yu. Sidorova, P. S. Kasatsky, E. A. Tolstyko, A. Paleskava, A. L. Konevega
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引用次数: 0
Abstract
Under adverse environmental conditions bacterial cells survive by activating the “stringent response,” which is mediated by the accumulation of small signaling alarmone molecules (p)ppGpp. The synthesis of (p)ppGpp from ATP and GDP/GTP and their hydrolysis to GDP/GTP and pyrophosphate are catalyzed by enzymes of the RSH (RelA/SpoT Homologue) family. Studying the complex allosteric mechanism underlying the bifunctional activity of these enzymes and the maintenance of intracellular levels of (p)ppGpp is a topical issue in modern science. In this work we determine the conformational stability and binding efficiency of the bifunctional homologue of the RSH family RelSeq385 with nucleotides depending on their concentration and the ionic composition of the reaction mixture.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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