Metabolomic Analysis of Both Microcystin-Producing and Microcystin-Free Microcystis aeruginosa Strains in Response to Exogenous Microcystin Exposure

IF 1.4 4区环境科学与生态学 Q4 WATER RESOURCES

Water SA Pub Date : 2025-03-28 DOI:10.3390/w17070993

Lijuan Cai, Chen Chen, Bingqing Wang, Gary Xie, Bo Wang, Xiuling Li, Wenxia Wang

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引用次数: 2

Abstract

Microcystis aeruginosa (M. aeruginosa), a key species in cyanobacterial blooms, is notably concerning due to its production of harmful microcystins (MCs). In this study, the differences in the ability of MC-producing and MC-free strains of M. aeruginosa to respond to the exogenous MCs (MC-LR) were compared. The results showed that at higher concentrations, MC-LR affected cell morphology, cell growth, photosynthetic efficiency, and induced oxidative stress in M. aeruginosa. Under high MC-LR concentration exposure, MC-producing strains showed a 14.7% reduction in cell density, accompanied by a 32% elevation in Vj and a 63.1% decline in FV/FM. MC-free strains showed cell density decreasing by 22.5%, Vj increasing 2-fold, and FV/FM dropping by 69.5%. The inhibitory effect of MC-LR at higher concentrations was found to be stronger in MC-free compared to MC-producing strains. In addition, MC-LR reduced the efficiency of photosystem II by blocking electron transfer from QA to QB; for MC-free strains, MC-LR may have acted as a signaling molecule affecting the targeting of QB on the D1 protein, thus leading to QB detachment from the protein complex. Metabolomics analysis showed that MC-LR affects arginine synthesis in M. aeruginosa and thus the synthesis and release of MCs.

查看原文本刊更多论文

产微囊藻毒素和不产微囊藻毒素的铜绿微囊藻菌株对外源性微囊藻毒素暴露的代谢组学分析

铜绿微囊藻（M. aeruginosa）是蓝藻华中的一个关键物种，由于其产生有害的微囊藻毒素（MCs）而备受关注。本研究比较了铜绿假单胞菌产生mc和不产生mc的菌株对外源mc （MC-LR）的反应能力差异。结果表明，高浓度MC-LR对铜绿假单胞菌的细胞形态、生长、光合效率产生影响，并诱导氧化应激。在MC-LR浓度较高的环境下，产mc菌株的细胞密度降低14.7%，Vj升高32%，FV/FM下降63.1%。无mc菌株细胞密度降低22.5%，Vj升高2倍，FV/FM下降69.5%。与产生mc的菌株相比，高浓度MC-LR对无mc菌株的抑制作用更强。此外，MC-LR通过阻断QA到QB的电子转移，降低了光系统II的效率；对于不含mc的菌株，MC-LR可能作为信号分子影响QB靶向D1蛋白，从而导致QB脱离蛋白复合物。代谢组学分析表明，MC-LR影响了M. aeruginosa的精氨酸合成，从而影响了MCs的合成和释放。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Water SA 环境科学-水资源

CiteScore

2.80

自引率

6.70%

发文量

审稿时长

18-36 weeks

期刊介绍： WaterSA publishes refereed, original work in all branches of water science, technology and engineering. This includes water resources development; the hydrological cycle; surface hydrology; geohydrology and hydrometeorology; limnology; salinisation; treatment and management of municipal and industrial water and wastewater; treatment and disposal of sewage sludge; environmental pollution control; water quality and treatment; aquaculture in terms of its impact on the water resource; agricultural water science; etc. Water SA is the WRC’s accredited scientific journal which contains original research articles and review articles on all aspects of water science, technology, engineering and policy. Water SA has been in publication since 1975 and includes articles from both local and international authors. The journal is issued quarterly (4 editions per year).