Isolation, identification and activity researching of ACC deaminase-containing rhizobacteria from lavender in Ili Kazakh Autonomous Prefecture

Abstract

Lavender is one of the important cash crops in Ili Kazakh Autonomous Prefecture. In this study, lavender rhizobacteria were isolated and purified, and their antibacterial activity was tested by filter paper method. The antioxidant activity and growth promotion ability were determined by DPPH, ABTS and PTIO assay as well as wheat seeds and rice seeds. The isolated strains were identified by morphological methods, physiological and biochemical experiments and molecular biological methods. Five strains of ACC deaminase-containing rhizobacteria were isolated, and four of them had antibacterial activity, and scavenging ability for DPPH, ABTS and PTIO free radicals, and has growth promotion effect on wheat and rice seedlings. Through 16sRNA sequence analysis, strains were identified as Pseudomonas rhizophila, Pseudomonas savastanoi, Klebsiella pasteurii, Klebsiella pneumoniae and Serratia plymuthica. In conclusion, this study provides a certain theoretical basis for the utilization of rhizobacteria in plant resources.