{"title":"Enhanced non-invasive extraction of proteinaceous binders from painted artifacts using composite gellan gum","authors":"Jin Dong, Zhanyun Zhu, Xiaoxi Li, Desheng Lan, Jianhua Huang, Ping Zhou","doi":"10.1038/s40494-025-01819-3","DOIUrl":null,"url":null,"abstract":"This study introduces a method for the non-invasive extraction of proteinaceous binders from painted artifacts using high-acyl gellan gums combined with urea, acetic acid, and Tris buffer. The aim is to preserve the integrity of artifacts while enhancing the efficiency of extracting aged proteins. The results demonstrate that gellan gum with urea has minimal impact on the macroscopic and microscopic surface properties, pH, contact angle, color difference, and internal structure of the painted artifacts. Additionally, the proteomic analysis is highly effective. This method offers the advantages of simple preparation and a concise sampling procedure, requiring only ten minutes for binder extraction. Given its non-invasive nature, this technique has the potential to become a valuable tool for non-invasive extraction of proteinaceous binders in painted artifacts.","PeriodicalId":19270,"journal":{"name":"npj Materials Degradation","volume":"13 1","pages":""},"PeriodicalIF":7.5000,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s40494-025-01819-3.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Materials Degradation","FirstCategoryId":"0","ListUrlMain":"https://doi.org/10.1038/s40494-025-01819-3","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 2
Abstract
This study introduces a method for the non-invasive extraction of proteinaceous binders from painted artifacts using high-acyl gellan gums combined with urea, acetic acid, and Tris buffer. The aim is to preserve the integrity of artifacts while enhancing the efficiency of extracting aged proteins. The results demonstrate that gellan gum with urea has minimal impact on the macroscopic and microscopic surface properties, pH, contact angle, color difference, and internal structure of the painted artifacts. Additionally, the proteomic analysis is highly effective. This method offers the advantages of simple preparation and a concise sampling procedure, requiring only ten minutes for binder extraction. Given its non-invasive nature, this technique has the potential to become a valuable tool for non-invasive extraction of proteinaceous binders in painted artifacts.
期刊介绍:
npj Materials Degradation considers basic and applied research that explores all aspects of the degradation of metallic and non-metallic materials. The journal broadly defines ‘materials degradation’ as a reduction in the ability of a material to perform its task in-service as a result of environmental exposure.
The journal covers a broad range of topics including but not limited to:
-Degradation of metals, glasses, minerals, polymers, ceramics, cements and composites in natural and engineered environments, as a result of various stimuli
-Computational and experimental studies of degradation mechanisms and kinetics
-Characterization of degradation by traditional and emerging techniques
-New approaches and technologies for enhancing resistance to degradation
-Inspection and monitoring techniques for materials in-service, such as sensing technologies