Enhanced non-invasive extraction of proteinaceous binders from painted artifacts using composite gellan gum

IF 7.5 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Jin Dong, Zhanyun Zhu, Xiaoxi Li, Desheng Lan, Jianhua Huang, Ping Zhou
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引用次数: 2

Abstract

This study introduces a method for the non-invasive extraction of proteinaceous binders from painted artifacts using high-acyl gellan gums combined with urea, acetic acid, and Tris buffer. The aim is to preserve the integrity of artifacts while enhancing the efficiency of extracting aged proteins. The results demonstrate that gellan gum with urea has minimal impact on the macroscopic and microscopic surface properties, pH, contact angle, color difference, and internal structure of the painted artifacts. Additionally, the proteomic analysis is highly effective. This method offers the advantages of simple preparation and a concise sampling procedure, requiring only ten minutes for binder extraction. Given its non-invasive nature, this technique has the potential to become a valuable tool for non-invasive extraction of proteinaceous binders in painted artifacts.
利用复合结冷胶增强从彩绘文物中非侵入性提取蛋白质粘合剂
本研究介绍了一种利用高酰基结冷胶结合尿素、乙酸和Tris缓冲液,从彩绘文物中无创提取蛋白质粘合剂的方法。目的是在提高提取老化蛋白质的效率的同时,保持人工制品的完整性。结果表明,添加尿素的结冷胶对涂层的宏观和微观表面性能、pH值、接触角、色差和内部结构的影响最小。此外,蛋白质组学分析是非常有效的。该方法制备简单,取样程序简洁,只需10分钟即可提取粘合剂。鉴于其非侵入性,该技术有可能成为一种有价值的工具,用于非侵入性提取彩色文物中的蛋白质粘合剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
npj Materials Degradation
npj Materials Degradation MATERIALS SCIENCE, MULTIDISCIPLINARY-
CiteScore
7.80
自引率
7.80%
发文量
86
审稿时长
6 weeks
期刊介绍: npj Materials Degradation considers basic and applied research that explores all aspects of the degradation of metallic and non-metallic materials. The journal broadly defines ‘materials degradation’ as a reduction in the ability of a material to perform its task in-service as a result of environmental exposure. The journal covers a broad range of topics including but not limited to: -Degradation of metals, glasses, minerals, polymers, ceramics, cements and composites in natural and engineered environments, as a result of various stimuli -Computational and experimental studies of degradation mechanisms and kinetics -Characterization of degradation by traditional and emerging techniques -New approaches and technologies for enhancing resistance to degradation -Inspection and monitoring techniques for materials in-service, such as sensing technologies
文献相关原料
原料信息
原料厂家
1
1.0 mol/L Tris buffer (pH 6.8)
2
22
iron trioxide
1309-37-1
¥21.00-¥16175.00
3
HA gellan gum
71010-52-1
¥38.00-¥24812.35
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