Polyploid Induction Enhances Secondary Metabolite Biosynthesis in Clausena lansium: Morphological and Metabolomic Insights

IF 4.5 2区农林科学 Q1 AGRONOMY

Agriculture-Basel Pub Date : 2025-07-21 DOI:10.3390/agriculture15141566

Yu Ding, Liangfang Wu, Hengxi Wei, Zhichun Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin, Zhike Zhang, Zhike Zhang, Zhike Zhang

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引用次数: 1

Abstract

Polyploidy in plants can enhance stress resistance and secondary metabolite production, offering potential benefits for Clausena lansium (L.) Skeel, a medicinally valuable species. However, systematic studies of polyploidy-induced morphological, anatomical, and metabolic changes in this species are lacking. This study aimed to induce and characterize polyploid C. lansium lines, assess ploidy-dependent variations, and evaluate their impact on bioactive metabolite accumulation. Three cultivars were hybridized, treated with colchicine, and bred, yielding 13 stable polyploid lines confirmed by flow cytometry and chromosome counting. The polyploids exhibited distinct traits, including larger pollen grains, altered leaf margins, increased leaflet numbers, enlarged guard cells with reduced stomatal density, and thicker leaf tissues. Metabolomic analysis revealed that tetraploids accumulated significantly higher levels of flavonoids, alkaloids, and phenolic acids compared to diploids, while triploids showed moderate increases. These findings demonstrate that polyploidization, particularly tetraploidy, enhances C. lansium’s medicinal potential by boosting pharmacologically active compounds. The study expands germplasm resources and supports the development of high-quality cultivars for pharmaceutical applications.

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多倍体诱导提高黄貂草次生代谢物的生物合成：形态学和代谢组学的见解

植物多倍体可以增强植物的抗逆性和次生代谢物的产生，对Clausena lanum （L.）具有潜在的利用价值。骷髅，一种有药用价值的物种。然而，对该物种多倍体诱导的形态、解剖和代谢变化的系统研究尚缺乏。本研究旨在诱导和鉴定多倍体lanum系，评估倍性依赖性变异，并评估其对生物活性代谢物积累的影响。将3个品种进行杂交，用秋水仙碱处理，经流式细胞术和染色体计数证实，得到13个稳定的多倍体系。多倍体表现出花粉粒较大、叶缘改变、小叶数增加、保卫细胞增大、气孔密度减小、叶组织较厚等特征。代谢组学分析显示，与二倍体相比，四倍体积累的黄酮类、生物碱和酚酸含量显著增加，而三倍体则有适度增加。这些发现表明，多倍体化，特别是四倍体化，通过增加药理活性化合物，增强了lanum的药用潜力。该研究扩大了种质资源，为优质药用品种的开发提供了支持。

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来源期刊

Agriculture-Basel Agricultural and Biological Sciences-Food Science

CiteScore

4.90

自引率

13.90%

发文量

1793

审稿时长

11 weeks

期刊介绍： Agriculture (ISSN 2077-0472) is an international and cross-disciplinary scholarly and scientific open access journal on the science of cultivating the soil, growing, harvesting crops, and raising livestock. We will aim to look at production, processing, marketing and use of foods, fibers, plants and animals. The journal Agriculturewill publish reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.