Targeted enrichment of bruceine D from Brucea javanica by molecularly imprinted nanomagnetic sorbents

IF 11.2 4区医学 Q1 CHEMISTRY, MEDICINAL

Chinese Herbal Medicines Pub Date : 2026-10-01 Epub Date: 2025-07-10 DOI:10.1016/j.chmed.2025.07.001

Qilei Chen, Yueming Huang, Tsz Ching Wong, Guanhua Huang, Yizhao Yang, Jianye Zhang, Hubiao Chen

{"title":"Targeted enrichment of bruceine D from Brucea javanica by molecularly imprinted nanomagnetic sorbents","authors":"Qilei Chen, Yueming Huang, Tsz Ching Wong, Guanhua Huang, Yizhao Yang, Jianye Zhang, Hubiao Chen","doi":"10.1016/j.chmed.2025.07.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to develop and validate a novel method for targeted enrichment of bruceine compounds, whose structural complexity hinders both synthesis and conventional natural extraction. As a case study, bruceine D, a major antitumor component from <em>Brucea javanica</em> (Yadanzi in Chinese), was selected for enrichment using magnetic nanoparticles (MNPs) functionalized with molecularly imprinted polymers (MIPs).</div></div><div><h3>Methods</h3><div>A comprehensive chemical profiling of the <em>B. javanica</em> extract was performed to identify bruceine D and its structural analogues. MNPs coated with bruceine D-MIPs (MMIPs) were synthesized and characterized using various techniques, including transmission electron microscopy to observe the morphology, scanning electron microscopy to analyze the surface structure, and Fourier-transform infrared spectroscopy to confirm the successful functionalization of MIPs on MNPs. The adsorption performance of MMIPs was assessed through static and dynamic adsorption experiments. Subsequently, the process was validated for selectivity, repeatability, and reusability. The adsorption data were modeled using the Freundlich isotherm and kinetic models to reveal the adsorption mechanism.</div></div><div><h3>Results</h3><div>Among multiple analogues, bruceine D was confirmed as a major constituent of the <em>B. javanica</em> extract. The MMIPs demonstrated superior morphology, crystallinity, and functional surface modification, leading to favorable adsorption characteristics. Compared to non-imprinted polymers, they exhibited significantly stronger adsorption intensity and selectivity for bruceine D due to a lower Freundlich isotherm constant (0.0418) and a higher imprinting factor (1.69). The MMIPs showed excellent repeatability with a low relative standard deviation (0.75%) and maintained high extraction efficacy across multiple cycles. The MMIPs successfully isolated bruceine D from <em>B. javanica</em> extract, achieving a remarkable recovery yield (39.50 µg/g).</div></div><div><h3>Conclusion</h3><div>This study demonstrated a novel application of molecular imprinting technology targeting bruceine compounds. In particular, the MMIP-based selective enrichment of bruceine D from <em>B. javanica</em> showed superior efficiency, selectivity, and adsorption capacity compared to conventional methods. This advancement holds significant implications for natural product isolation, offering a sustainable and green strategy in the pharmaceutical industry for the isolation of complex bioactive compounds for therapeutic use.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"18 4","pages":"Pages 1067-1078"},"PeriodicalIF":11.2000,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638425000838","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/10 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

This study aimed to develop and validate a novel method for targeted enrichment of bruceine compounds, whose structural complexity hinders both synthesis and conventional natural extraction. As a case study, bruceine D, a major antitumor component from Brucea javanica (Yadanzi in Chinese), was selected for enrichment using magnetic nanoparticles (MNPs) functionalized with molecularly imprinted polymers (MIPs).

Methods

A comprehensive chemical profiling of the B. javanica extract was performed to identify bruceine D and its structural analogues. MNPs coated with bruceine D-MIPs (MMIPs) were synthesized and characterized using various techniques, including transmission electron microscopy to observe the morphology, scanning electron microscopy to analyze the surface structure, and Fourier-transform infrared spectroscopy to confirm the successful functionalization of MIPs on MNPs. The adsorption performance of MMIPs was assessed through static and dynamic adsorption experiments. Subsequently, the process was validated for selectivity, repeatability, and reusability. The adsorption data were modeled using the Freundlich isotherm and kinetic models to reveal the adsorption mechanism.

Results

Among multiple analogues, bruceine D was confirmed as a major constituent of the B. javanica extract. The MMIPs demonstrated superior morphology, crystallinity, and functional surface modification, leading to favorable adsorption characteristics. Compared to non-imprinted polymers, they exhibited significantly stronger adsorption intensity and selectivity for bruceine D due to a lower Freundlich isotherm constant (0.0418) and a higher imprinting factor (1.69). The MMIPs showed excellent repeatability with a low relative standard deviation (0.75%) and maintained high extraction efficacy across multiple cycles. The MMIPs successfully isolated bruceine D from B. javanica extract, achieving a remarkable recovery yield (39.50 µg/g).

Conclusion

This study demonstrated a novel application of molecular imprinting technology targeting bruceine compounds. In particular, the MMIP-based selective enrichment of bruceine D from B. javanica showed superior efficiency, selectivity, and adsorption capacity compared to conventional methods. This advancement holds significant implications for natural product isolation, offering a sustainable and green strategy in the pharmaceutical industry for the isolation of complex bioactive compounds for therapeutic use.

查看原文本刊更多论文

分子印迹纳米磁性吸附剂富集鸦鸦药材中的马钱子碱D

本研究旨在开发和验证一种新的方法来靶向富集马钱子碱化合物，该化合物的结构复杂性阻碍了合成和传统的自然提取。以鸦嘴菜（Brucea javanica）中的主要抗肿瘤成分马钱子碱D为研究对象，采用分子印迹聚合物（MIPs）功能化的磁性纳米颗粒（MNPs）富集富集。通过对爪哇木提取物进行全面的化学分析，鉴定了马钱子碱D及其结构类似物。采用透射电镜观察形貌，扫描电镜分析表面结构，傅里叶变换红外光谱证实MMIPs在MNPs上的成功功能化，并对MMIPs进行了表征。通过静态和动态吸附实验对MMIPs的吸附性能进行了评价。随后，验证了该过程的选择性、可重复性和可重用性。采用Freundlich等温线和动力学模型对吸附数据进行了建模，揭示了吸附机理。在多种类似物中，马钱子碱D被证实是爪哇木提取物的主要成分。MMIPs表现出优异的形貌、结晶度和功能性表面修饰，从而具有良好的吸附特性。与非印迹聚合物相比，该聚合物具有较低的Freundlich等温线常数（0.041 8）和较高的印迹因子（1.69），具有较强的吸附强度和选择性。MMIPs具有良好的重复性，相对标准偏差低（0.75%），并且在多个循环中保持较高的提取效率。MMIPs成功地从爪哇提取物中分离出了马钱子碱D，获得了显著的回收率（39.50µg/g）。本研究展示了分子印迹技术在马钱子碱类化合物上的新应用。与传统富集方法相比，基于mmip的选择性富集爪哇花中马钱子碱D的效率、选择性和吸附能力均优于传统富集方法。这一进展对天然产物的分离具有重要意义，为制药工业分离用于治疗用途的复杂生物活性化合物提供了可持续和绿色战略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chinese Herbal Medicines CHEMISTRY, MEDICINAL-

CiteScore

4.40

自引率

5.30%

发文量

629

审稿时长

10 weeks

期刊介绍： Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.