SHED secretome hydrogel combined with α-mangostin for periodontal bone regeneration: In vivo study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2026-03-01 Epub Date: 2026-02-21 DOI:10.1016/j.jobcr.2026.101425

Mohammed Ahmed Aljunaid , Rini Devijanti Ridwan , Hendrik Setia Budi , Yuliati , Huda Rashad Qaid , Rubbig Diferly Mahendra , Chanaya Miranda Riveira , Abumabdian Nasar Aqrabi , Ananda Firman Putranto , Farouk Al-Ghazaly , Osamah Mohammed Al-Kawri

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引用次数: 0

Abstract

Objectives

Periodontitis is a long-term inflammatory condition that results in the gradual loss of bone tissue surrounding the teeth. Cells derived from the pulp of human exfoliated deciduous teeth, also known as SHED cells, are very valuable in regenerative medicine due to their ease of access, rapid multiplication, and the ability to develop into multiple cell types. Additionally, medicinal plants such as mangosteen, which are rich in α-mangostin, show promise for periodontitis treatment. This compound, possessing potent anti-inflammatory and antioxidant properties. The purpose of this study was to investigate the molecular mechanisms of SHED secretome-loaded hydrogel combined with α-mangostin on the periodontal bone regeneration processes in a rat model.

Material and method

Sixty Male Wistar rats (n = 12 per group) were divided into five group: Control positive, Control negative, Treatment I, Treatment II, and Treatment III. After treatment, rats were humanely euthanized at three distinct time points (7, 14, and 21 days). Mandibular sections were then collected and analyzed using immunohistochemistry to quantify the expression levels of TNF-α, IL-10, and BMP-2. One-way ANOVA and a post hoc test (P < 0.05) were used to assess the data.

Result

The result showed decrease in TNF-α expression, and a significant increase in the expression of IL-10 and BMP-2 at 7th, 14th, and 21 days.

Conclusion

The combination of hydrogel SHED secretome α-mangostin effectively modulates the inflammatory environment by reducing TNF-α level and enhancing IL-10 production, thereby promoting the expression of the osteogenic factor BMP-2. This finding suggests an effective strategy for periodontal bone regeneration processes.

查看原文本刊更多论文

SHED分泌组水凝胶联合α-山竹苷用于牙周骨再生的体内研究。

目的：牙周炎是一种长期的炎症，导致牙齿周围的骨组织逐渐丧失。从人类脱落的乳牙牙髓中提取的细胞，也被称为SHED细胞，由于其易于获取，快速增殖以及能够发育成多种细胞类型，在再生医学中非常有价值。此外，山竹等药用植物富含α-山竹苷，有望治疗牙周炎。这种化合物，具有有效的抗炎和抗氧化特性。本研究旨在探讨SHED负载水凝胶联合α-山竹苷对大鼠牙周骨再生的分子机制。材料与方法：雄性Wistar大鼠60只，每组12只，分为5组：对照阳性、对照阴性、处理1、处理2、处理3。治疗后，分别在7、14、21天对大鼠实施人道安乐死。收集下颌骨切片，采用免疫组化分析，量化TNF-α、IL-10和BMP-2的表达水平。单因素方差分析和事后检验(P)结果：第7、14、21天TNF-α表达降低，IL-10、BMP-2表达显著升高。结论：水凝胶联合SHED分泌组α-山竹苷可通过降低TNF-α水平，提高IL-10的产生，从而促进成骨因子BMP-2的表达，从而有效调节炎症环境。这一发现提示了牙周骨再生过程的有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.