Genome-wide study of the R2R3-MYB gene family and analysis of NoMYB90 promoting anthocyanin biosynthesis in watercress (Nasturtium officinale R.Br.)

IF 4.6 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2026-05-01 Epub Date: 2026-02-23 DOI:10.1016/j.plantsci.2026.113071

Suchong Deng, Qiang Ding, Xilin Hou

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Abstract

Watercress (Nasturtium officinale R.Br.) is a kind of aquatic or semi-aquatic vegetable, which is rich in a variety of metabolites beneficial to human body. The R2R3-MYB gene family plays a key role in the biosynthesis of metabolites such as anthocyanins, but has not been characterized in watercress. Here, we identified 118 R2R3-NoMYB genes in the watercress genome and classified them into 35 subgroups. High-light treatment simultaneously enhanced anthocyanin accumulation in both lateral stems and upper main stems and strongly up-regulated the expression of subgroup S6 member NoMYB90 as well as the anthocyanin biosynthetic structural genes. Subcellular localization showed that NoMYB90 was located in the nucleus and cell membrane. Transient overexpression of NoMYB90 in tobacco leaves promoted anthocyanin accumulation, confirming its role as a positive regulator of anthocyanin biosynthesis. Dual-luciferase assay showed that NoMYB90 transactivates the anthocyanin biosynthetic genes NoCHS, NoF3H, NoDFR, and NoUF3GT. This study presented the first genome-wide analysis of the R2R3-MYB family in watercress and identified NoMYB90 as a positive regulator of anthocyanin biosynthesis. The findings provided a reference for further investigations of R2R3-NoMYB genes and established a foundation for the study of anthocyanin biosynthesis in watercress.

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水田芥R2R3-MYB基因家族的全基因组研究及促进花青素生物合成的NoMYB90分析

西洋菜（Nasturtium officinale R.Br.）是一种水生或半水生蔬菜，富含多种对人体有益的代谢物。R2R3-MYB基因家族在花青素等代谢物的生物合成中起着关键作用，但在豆瓣菜中尚未被表征。我们在豆瓣菜基因组中鉴定出118个R2R3-NoMYB基因，并将其划分为35个亚群。强光处理同时增加了花青素在侧茎和上部主茎中的积累，并强烈上调了S6亚群成员NoMYB90和花青素生物合成结构基因的表达。亚细胞定位表明，NoMYB90位于细胞核和细胞膜内。烟草叶片中短暂过表达NoMYB90促进了花青素的积累，证实了其作为花青素生物合成的正调节因子的作用。双荧光素酶测定表明，NoMYB90可激活花青素生物合成基因NoCHS、NoF3H、NoDFR和NoUF3GT。本研究首次对豆瓣菜中的R2R3-MYB家族进行了全基因组分析，并鉴定出NoMYB90是花青素生物合成的正调节因子。研究结果为进一步研究R2R3-NoMYB基因提供了参考，并为豆瓣菜花青素的生物合成研究奠定了基础。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.