Adipose-derived mesenchymal stem cells-derived exosomes containing nano-pearl powder water-soluble matrix promote osteogenic differentiation of MC3T3-E1 cells.

IF 6.3 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2026-12-31 Epub Date: 2026-02-26 DOI:10.1080/15476278.2026.2630547

Ling Chen, QiuHua Mao, WenBai Zhang, YaNan Cheng

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引用次数: 0

Abstract

Objective: To explore the synergistic effect of nano-pearl powder (NPP) and adipose-derived stem cell exosomes (ADSC-Exos) on the osteogenic potential of MC3T3-E1 cells.

Methods: The water-soluble matrix of NPP (NPP-WSM) was extracted via freeze-drying, and ADSC-Exos were isolated by ultracentrifugation. NPP-WSM was incorporated into ADSC-Exos through co-incubation to generate NPP-WSM-Exos. MC3T3-E1 cells were treated with NPP-WSM or NPP-WSM-Exos. Cell proliferation and migration were evaluated using CCK-8 and wound-healing assays, respectively. Osteogenic differentiation was assessed by Alizarin Red S staining and alkaline phosphatase (ALP) activity. The expression of osteogenesis-related genes (COL1A1, RUNX2, OCN, and OPN) was measured by qPCR and Western blotting. Transcriptome sequencing (RNA-seq) was conducted to identify signaling pathways activated by NPP-WSM-Exos.

Results: NPP-WSM-Exos displayed distinct exosome morphology and biomarkers, confirming their successful preparation. Significantly, NPP-WSM-Exos enhanced the viability of MC3T3-E1 cells compared to NPP-WSM alone and upregulated the expression of osteogenic genes, including COL1A1, RUNX2, OCN, and OPN, at both the transcriptional and translational levels. Additionally, NPP-WSM-Exos strongly promoted mineralization, as evidenced by the increased calcification observed through Alizarin Red S staining, and elevated alkaline phosphatase (ALP) activity, indicating excellent potential for osteogenic differentiation. Transcriptome sequencing showed that NPP-WSM-Exos significantly enhanced the PI3K/AKT pathway in MC3T3-E1 cells, while protein level detection indicated that NPP-WSM-Exos could increase AKT phosphorylation levels and inhibit GSK3β activity to improve osteogenic efficiency.

Conclusion: The use of adipose-derived stem cell exosomes to encapsulate NPP-WSM can increase the utilization of WSM, promote the proliferation of MC3T3-E1, and enhance the osteogenic differentiation ability.

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含纳米珍珠粉水溶性基质的脂肪源性间充质干细胞外泌体促进MC3T3-E1细胞成骨分化。

目的：探讨纳米珍珠粉（NPP）与脂肪源性干细胞外泌体（ADSC-Exos）对MC3T3-E1细胞成骨潜能的协同作用。方法：采用冷冻干燥法提取NPP- wsm水溶性基质，采用超离心分离ADSC-Exos。通过共孵育将NPP-WSM纳入ADSC-Exos，生成NPP-WSM- exos。用NPP-WSM或NPP-WSM- exos处理MC3T3-E1细胞。分别用CCK-8和创面愈合法评估细胞增殖和迁移。采用茜素红S染色和碱性磷酸酶（ALP）活性测定成骨分化程度。采用qPCR和Western blotting检测成骨相关基因COL1A1、RUNX2、OCN、OPN的表达。通过转录组测序（RNA-seq）鉴定NPP-WSM-Exos激活的信号通路。结果：NPP-WSM-Exos显示出不同的外泌体形态和生物标志物，证实其制备成功。值得注意的是，与NPP-WSM单独相比，NPP-WSM- exos增强了MC3T3-E1细胞的活力，并在转录和翻译水平上调了成骨基因COL1A1、RUNX2、OCN和OPN的表达。此外，NPP-WSM-Exos强烈促进矿化，通过茜素红S染色观察到钙化增加，碱性磷酸酶（ALP）活性升高，表明具有良好的成骨分化潜力。转录组测序结果显示，NPP-WSM-Exos显著增强了MC3T3-E1细胞中PI3K/AKT通路，蛋白水平检测表明，NPP-WSM-Exos可以提高AKT磷酸化水平，抑制GSK3β活性，从而提高成骨效率。结论：利用脂肪源性干细胞外泌体包埋NPP-WSM可提高WSM的利用率，促进MC3T3-E1的增殖，增强成骨分化能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.