Integrating Genetic and Biotechnological Approaches to Enhance High-CBD Cannabis sativa L. Cultivation for Medicinal Purposes.

IF 3.2 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2026-07-01 Epub Date: 2026-02-25 DOI:10.1007/s12033-026-01559-0

Aran Sokuma, Tawan Chatsungnoen, Chanthana Susawaengsup, Krittiya Tongkoom, Prakash Bhuyar

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引用次数: 0

Abstract

The increasing demand for cannabidiol (CBD) and the relaxation of legal restrictions have driven the rapid expansion of cannabis research, cultivation, and commercialization. This review synthesizes current knowledge on optimizing high-CBD cannabis cultivation, focusing on genetic, environmental, and technological determinants of yield, cannabinoid biosynthesis, and compliance with regulatory frameworks. Key aspects include genetic stabilization, molecular breeding, and CRISPR-based gene editing to ensure consistency in cannabinoid profiles; precision agronomy practices such as fertigation, irrigation, and controlled-environment agriculture; and the role of abiotic stresses, light spectrum, temperature, and photoperiod in modulating plant growth and secondary metabolism. Furthermore, the paper evaluates regulatory landscapes across regions, strain-specific performance, and the integration of artificial intelligence in trait selection and production monitoring. By linking molecular genetics with cultivation systems and global trade patterns, this review highlights pathways to enhance yield stability, product quality, and medicinal efficacy of high-CBD cannabis. The study highlights the pressing need for scientifically informed cultivation practices, harmonized regulatory standards, and interdisciplinary innovation to promote cannabis as a sustainable agricultural and medicinal resource.

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整合遗传和生物技术方法促进高cbd大麻药用种植。

对大麻二酚（CBD）需求的增加和法律限制的放松推动了大麻研究、种植和商业化的迅速扩张。这篇综述综合了目前关于优化高cbd大麻种植的知识，重点是产量的遗传、环境和技术决定因素，大麻素生物合成，以及遵守监管框架。关键方面包括遗传稳定、分子育种和基于crispr的基因编辑，以确保大麻素谱的一致性；精准农艺实践，如施肥、灌溉和控制环境农业；以及非生物胁迫、光谱、温度和光周期对植物生长和次生代谢的调节作用。此外，本文还评估了跨区域的监管格局、菌株特异性表现以及人工智能在性状选择和生产监测中的整合。通过将分子遗传学与种植系统和全球贸易模式联系起来，本文综述了提高高cbd大麻产量稳定性、产品质量和药用功效的途径。该研究强调了迫切需要科学知情的种植实践、统一的监管标准和跨学科创新，以促进大麻作为可持续的农业和医药资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.