Neurofilament Light Chain Measurements by Centaur and Simoa Systems in Human and Murine Samples.

IF 0.8 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Israel Medical Association Journal Pub Date : 2026-02-01

Tali Drori, Amir Dori, Zehavit Goldberg, Valery Golderman, Polina Sonis, Michael Gurevich, Rina Zilkha-Falb, Joab Chapman, Efrat Shavit-Stein

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引用次数: 0

Abstract

Background: Neurofilament light chain (NfL) is an established biomarker for detecting axonal injury in various neurological disorders. The Quanterix Single Molecule Array (Simoa) is the current standard; however, automated immunoassays, such as the Siemens Atellica and Centaur, may serve as alternatives.

Objectives: To compare NfL measurements obtained with the Centaur system to those from the Simoa-SR-X. To assess their agreement and applicability in clinical practice, research, and animal studies.

Methods: NfL levels were measured in 27 human serum, 8 plasma, and 16 cerebrospinal fluid (CSF) samples, and 9 murine serum samples, by Centaur and Simoa systems. NfL levels in concomitantly drawn serum and plasma were compared in 8 humans. The agreement between platforms was evaluated.

Results: NfL levels measured by Centaur and Simoa systems demonstrated a strong correlation in serum (Spearman r=0.97, P < 0.0001) and plasma (Pearson R²=0.95, P < 0.0001). Centaur measurements were higher (P = 0.01) than Simoa. Most importantly, system-specific Z-scores corrected these differences. Serum and plasma levels measured by the Centaur system correlated strongly (R²=0.98, P < 0.0001) and showed similar results. CSF levels measured by the Centaur system were lower (52% bias) than those measured by Simoa, with poor correlation at concentrations within the normal range (R2=0.32, P = 0.11). Mouse serum results showed a strong correlation between systems (R²=0.86, P < 0.001) with similar values.

Conclusions: The Centaur system offers an alternative to Simoa for measuring NfL in human serum, plasma, and murine serum. System-specific age-adjusted Z-scores are essential for interpretation. CSF evaluation requires further assessment.

本刊更多论文

半人马和Simoa系统在人类和小鼠样本中的神经丝轻链测量。

背景：神经丝轻链（Neurofilament light chain, NfL）是一种检测各种神经系统疾病中轴突损伤的生物标志物。Quanterix单分子阵列（Simoa）是目前的标准；然而，自动免疫测定，如西门子Atellica和Centaur，可以作为替代方案。目的：比较半人马座系统和Simoa-SR-X系统获得的NfL测量结果。评估其在临床实践、研究和动物实验中的一致性和适用性。方法：采用Centaur和Simoa系统检测27份人血清、8份血浆、16份脑脊液（CSF）和9份鼠血清中NfL水平。同时抽取8人血清和血浆中NfL水平进行比较。对平台间的一致性进行了评估。结果：Centaur和Simoa系统测量的NfL水平在血清（Spearman r=0.97, P < 0.0001）和血浆（Pearson r²=0.95,P < 0.0001）中具有很强的相关性。Centaur的测量值高于Simoa （P = 0.01）。最重要的是，系统特定的z分数纠正了这些差异。Centaur系统测量的血清和血浆水平相关性强（R²=0.98,P < 0.0001），结果相似。Centaur系统测量的CSF水平比Simoa测量的低（52%偏差），在正常范围内浓度相关性较差（R2=0.32, P = 0.11）。小鼠血清结果显示，系统之间具有较强的相关性（R²=0.86,P < 0.001）。结论：Centaur系统为测量人血清、血浆和小鼠血清中的NfL提供了一种替代Simoa的方法。系统特定年龄调整z分数对于解释是必不可少的。脑脊液评估需要进一步评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Israel Medical Association Journal 医学-医学：内科

CiteScore

2.20

自引率

12.50%

发文量

审稿时长

3-8 weeks

期刊介绍： The Israel Medical Association Journal (IMAJ), representing medical sciences and medicine in Israel, is published in English by the Israel Medical Association. The Israel Medical Association Journal (IMAJ) was initiated in 1999.