Evaluation of host immune responses following immunization with MSP4 antigen adsorbed onto poly(lactic-co-glycolic acid) microparticles in a BALB/c murine model

IF 3.4 2区医学 Q2 INFECTIOUS DISEASES

Ticks and Tick-borne Diseases Pub Date : 2026-03-01 Epub Date: 2026-02-24 DOI:10.1016/j.ttbdis.2026.102619

Elsje Christine Rabie , Almudena González-García , Marinela Contreras , Mohamed Deifallah Yousif , José de la Fuente , Sudaxshina Murdan , Christian Stutzer , Christine Maritz-Olivier

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引用次数: 0

Abstract

Tick-borne diseases lead to millions of dollars in losses annually in livestock and associated industries. The tick-borne bacterium Anaplasma phagocytophilum causes diseases in most livestock and has demonstrated zoonosis. Currently there are no effective prophylaxes against this bacterium. MSP4 (major surface protein 4) has been identified as a promising vaccine candidate. Currently, data on the expression of this antigen in tick and host cells is lacking to establish it as an antigen accessible to the host immune response. When designing a vaccine against Anaplasma, it must be considered that the life cycle includes both free circulating and intracellular stages, and as such both Th1 and Th2 responses will most likely be needed for protection. This study provides evidence of the expression of MSP4 on tick and host cells, as well as T and B cell responses following immunisation with soluble and MSP4 coated PLGA microparticles in a murine model (BALB/c mice). This paves the way forward to rational improvement of vaccines against Anaplasma.

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在BALB/c小鼠模型中，MSP4抗原吸附在聚乳酸-羟基乙酸微颗粒上免疫后宿主免疫应答的评价

蜱传疾病每年给畜牧业和相关产业造成数百万美元的损失。蜱传细菌嗜吞噬细胞无原体在大多数牲畜中引起疾病，并已证实人畜共患病。目前还没有针对这种细菌的有效预防措施。MSP4（主要表面蛋白4）已被确定为有希望的候选疫苗。目前，缺乏该抗原在蜱虫和宿主细胞中表达的数据，无法将其确定为宿主免疫反应可及的抗原。在设计抗无原体疫苗时，必须考虑到生命周期包括自由循环和细胞内阶段，因此Th1和Th2反应很可能都需要保护。本研究提供了MSP4在蜱虫和宿主细胞上表达的证据，以及在小鼠模型（BALB/c小鼠）中用可溶性和MSP4包被PLGA微粒免疫后T细胞和B细胞反应的证据。这为合理改进抗无原体疫苗铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.