[Determination of the organ and species of lung tissue microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay].

Q4 Medicine

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2026-01-01 DOI:10.17116/sudmed20266901147

A A Gusarov, V L Sidorov, N E Surikova, O D Yagmurov, M N Nagornov

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引用次数: 0

Abstract

Objective: To determine the species belonging of lung tissue microparticles by total IgG using a quantitative enzyme-linked immunosorbent assay using the «IgG-total-ELISA-CHEMA» kit and to demonstrate the polymorphism of type I and II alveolocytes.

Material and methods: Microparticles of lung tissue taken from corpses were studied. The study of these samples was carried out using the forensic cytological method and the method of quantitative enzyme immunoassay using the «IgG-general-ELISA-CHEMA» test kit.

Results: The possibility of studying microparticles of lung tissue using ELISA has been established, optical density and total IgG concentrations have been determined, and optimal values for minimum and maximum calibration samples have been selected. Using the cytological method, the polymorphism of alveolocytes stained with fluorochromes and a neutral dye was demonstrated.

Conclusion: The data from the conducted high-tech research can be used to establish the species identity of microparticles of lung tissue found on instruments of injury.

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[用法医细胞学和定量酶联免疫吸附法测定肺组织微粒的器官和种类]。

目的：采用“IgG-total- elisa - chema”试剂盒，采用定量酶联免疫吸附法测定肺组织微粒中总IgG的种类，并证实I型和II型肺泡细胞的多态性。材料与方法：对尸体肺组织的微颗粒进行了研究。对这些样品的研究采用法医细胞学方法和定量酶免疫分析方法，使用«IgG-general-ELISA-CHEMA»检测试剂盒。结果：建立了利用ELISA法研究肺组织微颗粒的可能性，确定了光密度和总IgG浓度，选择了最小和最大校准样品的最佳值。利用细胞学方法，用荧光染料和中性染料染色肺泡细胞的多态性被证实。结论：该高科技研究数据可用于建立损伤器械上肺组织微粒的种类识别。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.