[Development of a method for laboratory diagnostics of acute oral FLY agaric poisoning by HPLC-MS/MS in urine analysis].

Q4 Medicine

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2026-01-01 DOI:10.17116/sudmed20266901122

E A Evdokimova, O L Balabanova, E A Kuznetsova, O Yu Strelova, A N Tursunov

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引用次数: 0

Abstract

Objective: To develop a method for laboratory diagnostics of acute poisoning with fly agarics using liquid tandem chromatograph mass spectrometry in urine analysis.

Material and methods: Identification was performed on a Nexera XR liquid chromatograph with an LCMS-8050 tandem mass spectrometer (Shimadzu, Japan) using a Shim-pack FC-ODS column, derivatization was performed by bimolecular dansylation. The object of the study was urine of patients of the Acute Poisoning Center of the State Budgetary Institution Research Institute of Emergency Medicine named after I.I. Dzhanelidze with a diagnosis of «Acute oral poisoning with fly agarics».

Results: It was established that urine is a suitable object for chemical-toxicological analysis in case of poisoning with fly agarics by the method of bimolecular dansylation, ion-chromatograms of ibotenic acid and muscimol, as well as a spectrum of ion-products of toxicants were obtained. The peak retention time (RT) for muscimol was 7.5 min, for ibotenic acid 6.25 min.

Conclusion: As a result of the study, a method for the identification of ibotenic acid and muscimol in urine using dansyl chloride derivatization by HPLC-MS/MS was developed.

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[尿液HPLC-MS/MS检测急性口服FLY木耳中毒的实验室诊断方法的建立]。

目的：建立尿液串联质谱法诊断急性木耳中毒的方法。材料和方法：采用Nexera XR液相色谱仪，LCMS-8050串联质谱（日本岛津），Shim-pack FC-ODS柱，双分子丹酰化衍生。研究对象为急诊医学国家预算机构研究所急性中毒中心（以I.I. Dzhanelidze命名）诊断为“急性口服木耳中毒”的患者尿液。结果：采用双分子丹酰化方法对蝇木耳中毒的尿液进行化学毒理学分析，获得了伊博烯酸和麝香酚的离子色谱图以及毒物的离子产物谱。muscimol的峰值保留时间为7.5 min， ibotenic acid的峰值保留时间为6.25 min。结论：本研究建立了氯丹酰衍生-高效液相色谱-质谱联用技术测定尿中伊博藤酸和麝香酚的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.