Effect of type I collagen on TLR-3 induced MMP-13 expression in human periodontal ligament fibroblasts

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2026-03-01 Epub Date: 2026-02-14 DOI:10.1016/j.jobcr.2026.101420

Ratthawut Namwad , Pitchaya Chaiyaraksa , Nirada Dhanesuan , Siriluck Tiranathanagul

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引用次数: 0

Abstract

Background

This study examined the effects of type I collagen, alone and in combination with poly I:C—a toll-like receptor 3 (TLR3) agonist—on matrix metalloproteinase-13 (MMP-13) expression and wound healing in human periodontal ligament (hPDL) fibroblasts.

Methods

hPDL fibroblasts were cultured and divided into four treatment groups: control, type I collagen (50 μg/mL), poly I:C (10 μg/mL), and a combination of type I collagen with poly I:C. Cytotoxicity was evaluated using the MTT assay. Cell migration was assessed via scratch assay at 0, 24, and 48 h. MMP-13 expression was quantified at both the mRNA and protein levels by real-time PCR and ELISA, respectively.

Results

After 24 h, the MTT assay indicated that none of the four treatment groups exhibited cytotoxicity toward hPDL fibroblasts. In the scratch assay at 24 and 48 h, type I collagen group demonstrated the fastest wound closure, whereas the poly I:C group showed the slowest migration. Regarding MMP-13 expression, the combination group displayed the highest mRNA levels, while ELISA revealed that both the combination and poly I:C groups had the greatest protein expression relative to the control.

Conclusion

This study provides evidence for an interaction between extracellular matrix signals and innate immune activation. Type I collagen promoted hPDL fibroblast migration, whereas poly I:C—a TLR3 agonist—upregulated MMP-13 expression, with the greatest effect observed in combination with collagen.

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I型胶原对TLR-3诱导的人牙周韧带成纤维细胞MMP-13表达的影响。

背景：本研究检测了I型胶原蛋白单独或联合poly I:C-a toll样受体3 （TLR3）激动剂对人牙周韧带（hPDL）成纤维细胞中基质金属蛋白酶-13 （MMP-13）表达和伤口愈合的影响。方法：培养hPDL成纤维细胞，将其分为对照组、I型胶原（50 μg/mL）、聚I:C （10 μg/mL）、I型胶原与聚I:C联合处理4组。采用MTT法评价细胞毒性。在0、24和48 h通过划痕法评估细胞迁移情况。通过实时荧光定量PCR和酶联免疫吸附法分别测定mRNA和蛋白水平的MMP-13表达。结果：24小时后，MTT实验显示，4个处理组均未表现出对hPDL成纤维细胞的细胞毒性。在24和48 h的划痕实验中，I型胶原组伤口愈合最快，而聚I:C组伤口迁移最慢。对于MMP-13的表达，联合组mRNA水平最高，而ELISA结果显示，联合组和聚I:C组相对于对照组蛋白表达最高。结论：本研究为细胞外基质信号与先天免疫激活之间的相互作用提供了证据。I型胶原促进了hPDL成纤维细胞的迁移，而poly I:C-a TLR3激动剂上调了MMP-13的表达，其中与胶原联合作用最大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.