Variant identification and genotyping strategy for the smg-1(r861) allele in Caenorhabditis elegans.

Abstract

The C. elegans smg-1 gene encodes a PI3K-related kinase responsible for initiating the process of nonsense-mediated decay (NMD). The smg-1 ( r861 ) allele is a strong loss-of-function variant that disrupts NMD activity leading to the stabilization of transcripts containing premature stop codons (PTCs). This allele has been used extensively in studies of RNA surveillance, transcript stability, and transgene regulation. Despite its widespread use, the mutation in smg-1 ( r861 ) has not been reported, and identification is often based solely on phenotype. Here, we identify the underlying mutation and present a restriction digestion-based genotyping strategy that enables quick confirmation of the smg-1 ( r861 ) allele.