Sonali Karhana, Yasmin Sultana, Nilanjan Saha, Nidhi, Mohd Ashif Khan
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引用次数: 0
Abstract
The aim of the study was to evaluate the angiogenic potential and safety of Dabrafenib, a BRAF inhibitor, for wound healing applications using the chick embryo Chorioallantoic Membrane (CAM) assay. Fertilized chicken eggs were incubated, and CAM assay was performed to assess angiogenesis. The number of blood vessels was quantified using Image J software. An irritation test was conducted by applying Dabrafenib to the CAM and observing vascular changes. Additionally, the effects of dabrafenib on the secretion of Vascular Endothelial Growth Factor (VEGF-A) and Fibroblast Growth Factor 2 (FGF2) in HaCaT cell lines were investigated. Dabrafenib demonstrated a dose-dependent pro-angiogenic effect, with a EC50 of 1.196 mM. The irritation test indicated no significant irritation with Dabrafenib. There was a 4.2-fold and 5.6-fold increase from control in the VEGF-A and FGF2 groups, respectively. Dabrafenib demonstrated angiogenic potential and was nonirritating, indicating its safety for topical application.
期刊介绍：
Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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