Tissue-Adhesive Chondroitin Sulfate Hydrogel for Cartilage Reconstruction

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS
Jisoo Shin, Eun Hye Kang, Soojeong Choi, Eun Je Jeon, Jung Ho Cho, Donyoung Kang, Hyungsuk Lee, In Sik Yun*, Seung-Woo Cho*
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引用次数: 34

Abstract

Chondroitin sulfate (CS), the main component of cartilage extracellular matrix, has attracted attention as a biomaterial for cartilage tissue engineering. However, current CS hydrogel systems still have limitations for application in successful cartilage tissue engineering owing to their unsuitable degradation kinetics, insufficient mechanical similarity, and lack of integration with the native cartilage tissue. In this study, using mussel adhesive-inspired catechol chemistry, we developed a functional CS hydrogel that exhibits tunable physical and mechanical properties as well as excellent tissue adhesion for efficient integration with native tissues. Various properties of the developed catechol-functionalized CS (CS-CA) hydrogel, including swelling, degradation, mechanical properties, and adhesiveness, could be tailored by varying the conjugation ratio of the catechol group to the CS backbone and the concentration of the CS-CA conjugates. CS-CA hydrogels exhibited significantly increased modulus (~10 kPa) and superior adhesive properties (~3 N) over conventional CS hydrogels (~hundreds Pa and ~0.05 N). In addition, CS-CA hydrogels incorporating decellularized cartilage tissue dice promoted the chondrogenic differentiation of human adipose-derived mesenchymal stem cells by providing a cartilage-like microenvironment. Finally, the transplantation of autologous cartilage dice using tissue-adhesive CS-CA hydrogels enhanced cartilage integration with host tissue and neo-cartilage formation owing to favorable physical, mechanical, and biological properties for cartilage formation. In conclusion, our study demonstrated the potential utility of the CS-CA hydrogel system in cartilage tissue reconstruction.

Abstract Image

用于软骨重建的组织黏附硫酸软骨素水凝胶
硫酸软骨素(CS)作为软骨细胞外基质的主要成分,作为软骨组织工程的生物材料备受关注。然而,目前的CS水凝胶体系由于降解动力学不合适、力学相似性不足、缺乏与天然软骨组织的整合,在成功的软骨组织工程中应用仍然存在局限性。在这项研究中,我们利用贻贝胶黏剂启发的儿茶酚化学,开发了一种功能性CS水凝胶,它具有可调的物理和机械性能,以及出色的组织粘附性,可与天然组织有效整合。通过改变儿茶酚基团与CS主链的共轭比和CS- ca偶联物的浓度,可以调整所制备的儿茶酚功能化CS (CS- ca)水凝胶的各种性能,包括溶胀、降解、力学性能和粘附性。与常规CS水凝胶(~ 100 Pa和~0.05 N)相比,CS- ca水凝胶的模量(~10 kPa)和粘附性能(~3 N)显著提高。此外,加入脱细胞软骨组织的CS- ca水凝胶通过提供软骨样微环境促进人脂肪源间充质干细胞的软骨分化。最后,使用组织粘胶CS-CA水凝胶移植自体软骨块,由于软骨形成的良好物理、力学和生物学特性,增强了软骨与宿主组织的整合和新软骨的形成。总之,我们的研究证明了CS-CA水凝胶系统在软骨组织重建中的潜在效用。
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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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