Assessing the reproductive potential of late mature cryopreserved oocytes

IF 3.9 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2026-03-01 Epub Date: 2025-08-20 DOI:10.1016/j.rbmo.2025.105231

Keri Bergin , Ashna Mehra , Rick Slifkin , Morgan Baird , Dmitry Gounko , Carlos Hernandez-Nieto , Joseph Lee , Chelsea Canon , Alan B. Copperman , Lucky Sekhon

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引用次数: 0

Abstract

Research question

Are there any differences in the warming, fertilization and embryo development outcomes of late mature cryopreserved oocytes versus immediately mature cryopreserved oocytes?

Design

This retrospective cohort study analysed autologous oocyte cryopreservation cycles with warming from January 2016 to September 2024. Group 1 comprised oocytes that were mature [metaphase II (MII)] at retrieval (assessed within 1 h of retrieval), and Group 2 comprised late mature oocytes, which included day 0 and day 1 late mature oocytes, which reached MII on re-assessment after short term (4–6 h) and next-day (18–28 h) rescue in-vitro maturation, respectively. Comparative statistics and univariate analysis were performed using Wilcoxon rank sum test and chi-squared test. Logistic regression fitted with a generalized estimating equation, adjusted for oocyte age, anti-Müllerian hormone, body mass index, year of oocyte cryopreservation and year of oocyte warming, was performed for warming survival, fertilization, blastulation and ploidy rates.

Results

Among 565 patients (699 cryopreservation and 629 warming cycles), Group 1 included 6899 oocytes that were mature at retrieval and Group 2 included 956 late mature oocytes. The warming survival rate was comparable between the groups (79.4% versus 78.0% for Groups 1 and 2, respectively; P = 0.33). The fertilization rate was significantly higher in Group 1 (75.3%) than Group 2 (59.8%) (adjusted OR 0.52, 95% CI 0.42–0.63; P < 0.001). Group 1 had significantly higher blastulation (37.7% versus 26.1%; P ≤ 0.001) and euploidy (54.5% versus 39.3%; P = 0.0023) rates compared with Group 2, and this was confirmed on adjusted analysis. A subanalysis showed no difference between day 0 and day 1 late mature oocytes.

Conclusions

Late mature cryopreserved oocytes show lower reproductive potential than oocytes that are mature at retrieval. This could be attributed to dysynchronous cytoplasmic and nuclear maturation. Despite suboptimal development, late mature oocytes can create high-quality blastocysts, offering additional opportunities for future family-building.

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评估冷冻保存的晚成熟卵母细胞的生殖潜力。

研究问题：晚成熟的冷冻保存卵母细胞与立即成熟的冷冻保存卵母细胞在升温、受精和胚胎发育结果上有什么不同？设计：本回顾性队列研究分析了2016年1月至2024年9月的自体卵母细胞低温保存周期。第1组为检索时成熟的卵母细胞[中期II (MII)]（检索后1小时内评估），第2组为晚期成熟卵母细胞，包括第0天和第1天的晚期成熟卵母细胞，分别在短期（4-6小时）和第二天（18-28小时）体外抢救成熟后重新评估达到MII。采用Wilcoxon秩和检验和卡方检验进行比较统计和单因素分析。对卵母细胞年龄、抗勒氏激素、体重指数、卵母细胞冷冻保存年份和卵母细胞加热年份进行校正，拟合了广义估计方程的Logistic回归，对加热存活率、受精率、囊胚率和倍性率进行了校正。结果：565例患者（699例冷冻保存，629例升温循环）中，1组有6899例取卵时成熟的卵母细胞，2组有956例晚成熟卵母细胞。各组间的升温存活率具有可比性（第一组为79.4%，第二组为78.0%;P = 0.33）。1组受精率（75.3%）显著高于2组（59.8%）（校正OR 0.52, 95% CI 0.42 ~ 0.63; P < 0.001）。组1的囊胚率（37.7% vs . 26.1%, P≤0.001）和整倍体率（54.5% vs . 39.3%, P = 0.0023）显著高于组2，经校正分析证实。亚分析显示第0天和第1天晚成熟卵母细胞之间没有差异。结论：较晚成熟的冷冻保存卵母细胞的生殖潜能低于在取出时成熟的卵母细胞。这可能归因于细胞质和核成熟不同步。尽管发育不理想，但成熟较晚的卵母细胞可以产生高质量的囊胚，为未来的家庭建设提供了额外的机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.