Exploring the bioactive potential of bovine pericardium membrane combined with hyaluronic Acid: characterization and cellular viability analyses

IF 3.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI:10.1016/j.bbrep.2026.102490

Dwi Wahyu Indrawati , Ernie Maduratna Setiawatie , Retno Pudji Rahayu , Rizky Briliant Syah Manurung , Mohammed Ahmed Aljunaid

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引用次数: 0

Abstract

This study explores the bioactive potential of bovine pericardium membranes combined with hyaluronic acid (HA, 120 kDa) at concentrations of .5%, 1.0%, and 2.0% through physicochemical characterization and fibroblast cell viability analysis. A laboratory experimental design was employed to evaluate bovine pericardium membranes modified with hyaluronic acid at different concentrations. Material characterization was conducted employing Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) analyses. Fibroblast cell viability was assessed using the MTT assay, and statistical analysis was conducted using the ANOVA test. The results demonstrated that hyaluronic acid modification altered the physicochemical characteristics of the membrane, including increased surface hydrophilicity. The cell viability test revealed comparable fibroblast viability among HA-modified membranes, indicating no cytotoxic effects. FTIR and SEM analyses confirmed chemical interactions and morphological features associated with favorable cell–material interactions. These findings suggest that bovine pericardium membrane with hyaluronic acid 120 kDa exhibit favorable physicochemical characteristics and in vitro cytocompatibility, indicating their potential for tissue engineering applications. This research provides a scientific foundation for developing innovative biomaterials that support favorable cell–material interactions for tissue regeneration research, contributing to advancements in tissue regeneration therapy.

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探索牛心包膜与透明质酸结合的生物活性潜力：表征和细胞活力分析

本研究探讨了牛心包膜与透明质酸（HA, 120 kDa）结合的生物活性潜力。5%， 1.0%和2.0%通过理化表征和成纤维细胞活力分析。采用室内实验设计对不同浓度透明质酸修饰的牛心包膜进行了评价。采用傅里叶变换红外光谱（FTIR）、扫描电镜（SEM）和x射线衍射（XRD）分析对材料进行了表征。采用MTT法评估成纤维细胞活力，采用方差分析进行统计分析。结果表明，透明质酸修饰改变了膜的物理化学特性，包括增加表面亲水性。细胞活力测试显示，ha修饰膜的成纤维细胞活力相当，表明没有细胞毒性作用。FTIR和SEM分析证实了与有利的细胞-物质相互作用相关的化学相互作用和形态特征。这些结果表明，含有透明质酸120 kDa的牛心包膜具有良好的理化特性和体外细胞相容性，具有组织工程应用的潜力。本研究为创新生物材料的开发提供了科学基础，为组织再生研究提供了有利的细胞-材料相互作用，有助于组织再生治疗的进步。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.