Colorimetric/fluorescent dual-module bivalent aptasensor for kanamycin detection based on silver nanoparticles and silver nanoclusters

IF 7 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control Pub Date : 2026-06-01 Epub Date: 2026-01-29 DOI:10.1016/j.foodcont.2026.112018

Dong Xu , Xingxing Li , Xinxin Yan , Lei Shan , Fuhou Li , Weixia Wang , Yuchengmin Zhang , Jinri Chen

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Abstract

Excessive intake of kanamycin (KAN) poses significant risks to human health, so it is necessary to develop rapid, portable, low-cost, and highly sensitive biosensors to monitor KAN. Herein, a colorimetric/fluorescent bivalent aptasensor for rapid detection of KAN was developed based on bivalent aptamer-templated silver nanoparticles (BApt-templated AgNPs) and bivalent aptamer-templated silver nanoclusters (BApt-AgNCs), which was constructed by cDNA-BApt-cDNA, NaBH₄ and AgNO₃. BApt-templeted AgNPs and BApt-AgNCs can be synthesized by changing the reaction temperature and time of NaBH₄ and AgNO₃ with cDNA-BApt-cDNA. This aptasensor is composed of a colorimetric/fluorescence dual detection module, and it supports absorbance/RGB/fluorescence three-mode detection. An ultralow detection limit of 5.98 fM was achieved in the fluorescence detection module and a detection range of 0–500 μM was achieved in the colorimetric detection module. Meanwhile, the aptasensor demonstrates excellent repeatability in actual sample testing, thus having great potential for on-site quantitative analysis and portable detection of KAN.

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基于纳米银和纳米银团簇的卡那霉素比色/荧光双模二价适体传感器

卡那霉素（kanamycin， KAN）的过量摄入对人体健康有重大危害，因此有必要开发快速、便携、低成本、高灵敏度的生物传感器来监测KAN。本文基于二价适配体模板银纳米粒子（BApt-templated AgNPs）和二价适配体模板银纳米团簇（BApt-AgNCs），构建了一种用于KAN快速检测的比色/荧光二价适配体传感器，该传感器由cDNA-BApt-cDNA、NaBH4和AgNO3构建。通过改变NaBH4和AgNO3与cDNA-BApt-cDNA的反应温度和时间，可以合成bapt - AgNPs和BApt-AgNCs。该适体传感器由比色/荧光双检测模块组成，支持吸光度/RGB/荧光三模式检测。荧光检测模块实现了5.98 fM的超低检测限，比色检测模块实现了0 ~ 500 μM的检测范围。同时，该传感器在实际样品检测中具有良好的重复性，在KAN的现场定量分析和便携式检测方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.