In vitro maturation of Nile tilapia (Oreochromis niloticus) testicular spermatozoa in different media considering with motility, oxidative stress, fatty acid profile and fertilization capacity

IF 2.9 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2026-05-01 Epub Date: 2026-02-07 DOI:10.1016/j.theriogenology.2026.117860

Burak Evren İnanan

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引用次数: 0

Abstract

Collection and usage of testicular spermatozoa are often required for reproduction practices due to low sperm volume, reduced sperm density, urine contamination, and utilization of spermatozoa of sex-reversed broodstocks. No previous study has investigated in vitro maturation (IVM) of Nile tilapia (Oreochromis niloticus) testicular spermatozoa, despite being a widely cultured species in the world. The main aim of the present study was to establish an IVM protocol for testicular spermatozoa of this species. Accordingly, testicular sperm samples were first diluted with four different media including Leibovitz L15 Medium (LE15), and monitored in terms of their motility parameters at 4 and 28 °C. After determination of the optimum medium (as LE15) and the most appropriate temperature condition (as the first 3 h at 28 °C and followed by incubation at 4 °C), the changes in oxidative condition as well as fertilization and hatching rates were determined. Motility parameters, oxidative status and fatty acid profiles of the testicular spermatozoa improved with this IVM protocol compared with those from fresh and undiluted testicular spermatozoa. Fertilization and hatching rates were found as 92 % and 75 % with stripped spermatozoa, 58 % and 47 % with fresh testicular spermatozoa, 17 % and 11 % for undiluted testicular spermatozoa, 76 % and 63 % for in vitro matured spermatozoa, respectively. The results of the study provide useful information on the usage of testicular spermatozoa for propagation in Nile tilapia.

查看原文本刊更多论文

尼罗罗非鱼（Oreochromis niloticus）睾丸精子在不同培养基中体外成熟的动力学、氧化应激、脂肪酸谱和受精能力。

由于精子数量少、精子密度低、尿液污染和利用性别逆转的亲鱼的精子，在生殖实践中经常需要收集和使用睾丸精子。尼罗罗非鱼（Oreochromis niloticus）是世界上广泛养殖的一种鱼类，但目前尚无研究对其睾丸精子的体外成熟（IVM）进行研究。本研究的主要目的是建立该物种睾丸精子的体外受精方案。因此，首先用莱博维茨L15培养基（LE15）等四种不同的培养基稀释睾丸精子样本，并在4°C和28°C下监测其运动参数。在确定最佳培养基（LE15）和最适宜的温度条件（28℃前3 h， 4℃孵育）后，测定氧化条件的变化以及受精率和孵化率。与新鲜和未稀释的睾丸精子相比，该IVM方案改善了睾丸精子的运动参数、氧化状态和脂肪酸谱。剥去精子的受精率为92%和75%，新鲜精子的受精率为58%和47%，未稀释睾丸的受精率为17%和11%，体外成熟精子的受精率为76%和63%。研究结果为尼罗罗非鱼睾丸精子的繁殖提供了有益的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.