TgARAD1 regulated by TgERF8 enhances the tolerance of Torreya grandis to drought stress

IF 4.6 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI:10.1016/j.plantsci.2026.113047

Ruoman Wang , Tongtong Wang , Ya Liu , Jin Su , Hangbiao Jin , Jiasheng Wu , Jingwei Yan

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引用次数: 0

Abstract

Upon exposure to drought conditions, plants rapidly shut their stomata to minimize water loss-a response critically influenced by the cell wall properties of guard cells. The ARABINAN DEFICIENT (ARAD) gene family modulates cell wall composition and architecture by regulating the biosynthesis of arabinan, a key pectic side chain. While ARAD1 has been implicated in plant growth and development, its function in abiotic stress responses remains poorly understood. Here, the expression of TgARAD1 in Torreya grandis, a gymnosperm species with extremely high medicinal value, was significantly upregulated under drought stress. Subcellular localization experiment demonstrated that TgARAD1 was resided in the Golgi apparatus. Overexpression of TgARAD1 promoted stomatal closure, reduced water loss, enhanced antioxidant defense and mitigated oxidative damage, ultimately enhancing drought tolerance in Arabidopsis thaliana. Further study revealed that the transcription factor TgERF8 activated the expression of TgARAD1 through direct interaction with its promoter. Taken together, our findings uncover a previously uncharacterized drought-responsive regulatory module in which TgERF8 enhances TgARAD1 expression to modulate stomatal closure and oxidative stress resistance. This investigation offers new viewpoint into the pivotal role of ARAD in via adaptation through stomatal regulation and transcriptional control.

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TgERF8调控的TgARAD1增强了香榧对干旱胁迫的耐受性。

在暴露于干旱条件下，植物迅速关闭气孔以减少水分流失，这一反应受到保护细胞细胞壁特性的严重影响。ARABINAN缺陷（ARAD）基因家族通过调节关键果胶侧链ARABINAN的生物合成来调节细胞壁的组成和结构。虽然ARAD1与植物生长发育有关，但其在非生物胁迫反应中的功能尚不清楚。结果表明，TgARAD1在具有极高药用价值的裸子植物大香榧中表达量在干旱胁迫下显著上调。亚细胞定位实验表明，TgARAD1存在于高尔基体中。TgARAD1的过表达促进了拟南芥的气孔关闭，减少了水分流失，增强了抗氧化防御，减轻了氧化损伤，最终提高了拟南芥的耐旱性。进一步研究发现，转录因子TgERF8通过与其启动子的直接相互作用激活TgARAD1的表达。综上所述，我们的研究结果揭示了一个以前未被描述的干旱响应调节模块，其中TgERF8增强TgARAD1的表达来调节气孔关闭和氧化应激抗性。该研究为研究ARAD通过气孔调节和转录调控在植物适应性中的关键作用提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.