Similar developmental outcomes of salamander larvae produced with fresh and cryopreserved sperm: implications for amphibian conservation

IF 2.6 3区生物学 Q2 BIOLOGY

Cryobiology Pub Date : 2026-03-01 Epub Date: 2026-02-10 DOI:10.1016/j.cryobiol.2026.105593

Shaina Lampert , Isabella Burger , Devin Chen , Andy Kouba , Carrie Kouba

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Abstract

Sperm cryopreservation is an important tool for the genetic management of threatened amphibian species within conservation breeding programs. Yet, little is known about the growth and development of offspring generated using frozen-thawed sperm compared to those produced using fresh sperm. The objective of this study was to compare the fertilization rate, embryo development, morphology, and survival of tiger salamander (Ambystoma tigrinum) offspring generated using either fresh or frozen-thawed sperm. Captive male and female A. tigrinum were administered exogenous hormones to stimulate gamete production and release. Eggs were collected from females and fertilized with either freshly collected sperm from captive males or frozen-thawed sperm maintained in the U.S. National Amphibian Genome Bank. Following in-vitro fertilization, egg fertilization rates and early embryo development were monitored through hatching. Larvae from each sperm type were then maintained under standardized rearing conditions and routinely monitored for total length, head width, survival, and development through metamorphosis. Results showed no difference in larval growth (p > 0.42), survival rate (p = 0.72), or size at metamorphosis (p > 0.26) between offspring generated from fresh or frozen-thawed sperm. These findings support sperm cryopreservation as an effective conservation tool for producing viable individuals in amphibian conservation programs.

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用新鲜和冷冻保存的精子产生的蝾螈幼虫的相似发育结果：两栖动物保护的意义。

精子冷冻保存是保护繁殖计划中濒危两栖动物遗传管理的重要手段。然而，与使用新鲜精子产生的后代相比，使用冷冻解冻精子产生的后代的生长发育情况鲜为人知。本研究的目的是比较鲜精和冻融精对虎蝾螈（Ambystoma tigrinum）后代的受精率、胚胎发育、形态和存活率。用外源激素刺激捕获的雄性和雌性刺槐产生和释放配子。从雌性身上收集卵子，并与从圈养雄性身上收集的新鲜精子或保存在美国国家两栖动物基因组库中的冷冻解冻精子受精。体外受精后，通过孵化监测卵子受精率和早期胚胎发育情况。然后将每种精子类型的幼虫置于标准化饲养条件下，并定期监测其总长度、头宽、存活率和变态发育情况。结果显示，鲜精子和冻融精子产生的后代在幼虫生长（p > 0.42）、存活率（p = 0.72）和变形时的大小（p > 0.26）方面没有差异。这些发现支持精子冷冻保存作为一种有效的保护工具，在两栖动物保护计划中产生可行的个体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryobiology 生物-生理学

CiteScore

5.40

自引率

7.40%

发文量

审稿时长

56 days

期刊介绍： Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.