Selina Uhlendorff, Prof. Dr. Ulrich Odefey, Prof. Dr. Björn Frahm, Prof. Dr. Miriam Pein-Hackelbusch
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引用次数: 0
Abstract
Determining cell density and viability with (semi-)automated methods enables rapid cell cultivation analysis. However, scientific validation is required, typically by comparing results to manual counting. To address this, we present a method to evaluate and compare two cell counting methods exemplified by a manual and a semi-automated method (Countstar BioTech). Experiments followed validation parameters aligned with the International Council for Harmonization (ICH) Q2(R1) guideline and a dilution series design based on ISO 20391-2:2019. Both the semi-automated and manual methods showed comparable specificity and linearity for Chinese hamster ovary (CHO)-K1 cells. The semi-automated method exhibited superior repeatability for total cell density, whereas cell viability results showed no significant difference.
期刊介绍：
Die Chemie Ingenieur Technik ist die wohl angesehenste deutschsprachige Zeitschrift für Verfahrensingenieure, technische Chemiker, Apparatebauer und Biotechnologen. Als Fachorgan von DECHEMA, GDCh und VDI-GVC gilt sie als das unverzichtbare Forum für den Erfahrungsaustausch zwischen Forschern und Anwendern aus Industrie, Forschung und Entwicklung. Wissenschaftlicher Fortschritt und Praxisnähe: Eine Kombination, die es nur in der CIT gibt!
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