Molecular surveillance and genetic diversity of Enterohemorrhagic Escherichia coli O157:H7 Pathotypes using MLVA method

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2026-05-01 Epub Date: 2026-02-02 DOI:10.1016/j.rvsc.2026.106093

Sedigheh Mokhtari , Yahya Tahamtan , Mohammad Kargar , Keyvan Tadayon , Elham Moazamian

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引用次数: 0

Abstract

Background

Escherichia coli O157 is a major foodborne pathogen associated with severe human disease, with cattle serving as its primary reservoir, and in this study multilocus variable-number tandem-repeat analysis (MLVA) was applied to investigate the genetic diversity, distribution, and population structure of isolates collected in Fars Province, Iran.

Method

This study was performed on 150 swab samples were collected from rectum and carcasses of cattle, sheep and goats and fruit juice in Fars province. Samples were processed following standard bacteriological procedures. MLVA method was performed based on polymerase chain reaction (PCR) by using four variable-number tandem repeat (VNTR).

Results

22 isolates were identified as E. coli O157:H7 that 15, 6 and 1 were from cattle, sheep and fruit juice, respectively. By using MLVA technique, 22 isolates were separated into 15 distinct MLVA types. MST algorithm showed five genetically related clusters and by UPGMA method isolates were classified in 3 main cluster and 8 sub clusters.

Conclusion

This study showed that MLVA can be used as a valid, inexpensive and rapid method to trace and analyze the phylogenetic relationships between E. coli strains in epidemiological research.

查看原文本刊更多论文

应用MLVA法监测肠出血性大肠杆菌O157:H7病原型的分子监测和遗传多样性。

背景：大肠杆菌O157是一种与人类严重疾病相关的主要食源性病原体，牛是其主要宿主，本研究采用多位点可变数串联重复分析（MLVA）研究了在伊朗法尔斯省收集的分离株的遗传多样性、分布和种群结构。方法：对法尔斯省牛、绵羊、山羊直肠和尸体的150份拭子样本和果汁进行研究。样品按照标准细菌学程序进行处理。采用四可变数串联重复序列（VNTR），采用聚合酶链反应（PCR）进行MLVA检测。结果：鉴定出22株O157:H7大肠杆菌，其中牛、羊和果汁分别分离出15株、6株和1株。利用MLVA技术将22株分离得到15个不同的MLVA型。MST算法显示5个遗传相关簇，UPGMA方法将分离物分为3个主簇和8个亚簇。结论：在流行病学研究中，MLVA可作为一种有效、廉价、快速的大肠杆菌菌株系统发育关系追踪分析方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.