Multi-omics profiling uncovers metabolic regulation in cumulus cells during oocyte maturation

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2026-05-01 Epub Date: 2026-02-03 DOI:10.1016/j.anireprosci.2026.108121

Yassin Kassim , Hao Sheng , Haibin Zhong , Mostafa Elashry , Omaima Mohamed Kandil , Esraa Aly Ismail , Kun Zhang

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Abstract

Bidirectional signalling between the oocyte and surrounding cumulus cells is essential for coordinating follicular development and acquiring oocyte competence. The metabolic networks governing bovine cumulus cell function, however, remain poorly characterized. To address this gap, we performed integrated metabolomic and proteomic analyses of cumulus cells isolated from cumulus-oocyte complexes at two critical maturation stages: (1) meiotically arrested germinal vesicle stage (0 h) and (2) metaphase II stage (24 h). This temporal profiling reveals dynamic metabolic reprogramming events supporting oocyte maturation. In particular, we found the active use of glucose metabolism via glycolysis and the pentose phosphate pathway, rather than the citric cycle, suppression in fatty acid β-oxidation, and active tryptophan and polyamine biosynthesis. Meanwhile, the surrounding cumulus cells showed a steady rise in steroid hormone production and one-carbon metabolism in tandem with the development of the oocyte. In sum, the data provide a comprehensive metabolic atlas of cumulus cell differentiation and give prospective indicators for enhancing and predicting oocyte quality.

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多组学分析揭示了卵母细胞成熟过程中积云细胞的代谢调节

卵母细胞和周围卵丘细胞之间的双向信号传导是协调卵泡发育和获得卵母细胞能力的必要条件。然而，控制牛积云细胞功能的代谢网络的特征仍然很差。为了解决这一差距，我们对两个关键成熟阶段的卵丘细胞进行了综合代谢组学和蛋白质组学分析：(1)减数分裂阻滞的生发囊泡期（0 h）和(2)中期II期（24 h）。这种时间分析揭示了支持卵母细胞成熟的动态代谢重编程事件。特别是，我们发现通过糖酵解和戊糖磷酸途径而不是柠檬酸循环积极利用葡萄糖代谢，抑制脂肪酸β-氧化，活跃色氨酸和多胺的生物合成。同时，随着卵母细胞的发育，周围的积云细胞类固醇激素的产生和单碳代谢稳步上升。总之，这些数据为卵丘细胞分化提供了一个全面的代谢图谱，为提高和预测卵母细胞质量提供了前瞻性指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.